Method for distinguishing fungi and bacteria

Abstract

The invention discloses a method for distinguishing fungi and bacteria, which comprises the following steps of: detecting whether the bacteria to be detected can induce the compounds expressed by the formulas (I and II) to cause fluorescence resonance energy transfer; if the bacteria to be detected can induce the compounds expressed by the formulas (I and II) to cause fluorescence resonance energy transfer, determining that the bacteria to be detected is bacteria; and if the bacteria to be detected cannot induce the compounds expressed by the formulas (I and II) to cause fluorescence resonance energy transfer, determining that the bacteria to be detected is fungi. The method has the advantages of high speed, visibility and convenience and the like. The method can be used for distinguishing the types of the infectious pathogenic bacteria, instructing the administration of the doctors aiming at different patients, improving the therapeutic efficiency and reducing the occurrence of drug resistance caused by incorrect use of antibiotic. The method also has application value in aspects of food safety, environmental monitoring and the like.

Description

technical field The present invention relates to a method for differentiating fungi and bacteria. Background technique The detection of pathogenic bacteria is of great significance in the fields of medical treatment, food safety and environmental science. Since deep fungal infections are occult and have no characteristic clinical manifestations, the quick distinction between fungi and bacteria has important guiding significance for physicians to administer more targeted drugs clinically. In deep fungal infections, Candida albicans is the most common pathogen, accounting for about 40% of all types of fungi; while in blood-borne infections caused by bacteria, Escherichia coli is the most common type of bacteria, among which E.coli O157:H7 occupies a considerable proportion in various bacterial infections. At present, the traditional methods for the detection of pathogenic bacteria mainly include microbial culture methods, membrane filtration methods, and biochemical detecti...

AI Technical Summary

Problems solved by technology

Although the existing detection methods have certain sensitivity and selectivity, the shortcomings of high cost, complicated preparation, long-term processing and special requirements for instruments limit the large-scale application of these methods

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