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O type foot-and-mouth disease virus variant as well as coding gene and application thereof

A technology of foot-and-mouth disease virus and mutant strains, which is applied in the fields of application, virus peptide, gene therapy, etc., and can solve the problems of accelerated mutation of foot-and-mouth disease virus

Inactive Publication Date: 2010-09-22
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Under the strong and continuous immune pressure, the mutation rate of foot-and-mouth disease virus is greatly accelerated, which brings new challenges to the prevention and control of the disease

Method used

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  • O type foot-and-mouth disease virus variant as well as coding gene and application thereof
  • O type foot-and-mouth disease virus variant as well as coding gene and application thereof
  • O type foot-and-mouth disease virus variant as well as coding gene and application thereof

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Experimental program
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Embodiment

[0029] 1. Experimental materials and methods

[0030] 1.1 Cells, viruses and antibodies

[0031] BHK-21 cells (purchased from ATCC, USA) were cultured in DMEM containing 10% fetal bovine serum, in 5% CO 2 cultured in a 37°C incubator. The isolated O-type foot-and-mouth disease virus O / YS / CHA / 05 strain was propagated in DMEM medium containing 2% fetal bovine serum, and the virus was harvested when 80% of the cells appeared CPE. After repeated freezing and thawing three times, 2000 × g, (4°C) centrifugation for 10 min, and the supernatant was stored at -70°C. E. coli competent DH5a and BL21(DE3) were preserved by our laboratory, and pGEX-6p-1 vector was purchased from Promega Company. The pOK12 vector was donated by Messing (GenBank accession number AF223639), and the T7 promoter sequence of the vector was removed by double digestion with EcoRV and FspI in the present invention.

[0032] The monoclonal antibody 8E8 of the O-type FMDV O / YS / CHA / 05 strain (the hybridoma cell li...

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Abstract

The invention discloses an O type foot-and-mouth disease virus variant as well as a coding gene and application thereof. In the invention, an O type foot-and-mouth disease virus pan-Asia strain O / YS / CHA / 05 is firstly separated out, the nucleotide sequence of the O type foot-and-mouth disease virus pan-Asia strain O / YS / CHA / 05 SEQ ID NO: 1, and the amino acid sequence is SEQ ID NO: 2. In comparison with a VP1 amino acid sequence, the virus strain has 7 variable sites, five of which are centralized in a G-H ring. Mutation of the sites ensures that the virus variant has the capability of escaping from host immunity so as to have the superiority for becoming a popular virus strain. Therefore, the variant can be employed to prepare an inactivated vaccine for prevention and treatment of the variant and relevant strains, dominant antigen epitope of the variant can be employed to prepare a synthetic peptide vaccine, and the variant can be employed to develop novel O type foot-and-mouth disease virus vaccines such as VLP vaccine and the like. Therefore, the invention has important value in controlling the popularity of O / YS / CHA / 05 and relevant variable strains.

Description

technical field [0001] The present invention relates to O-type foot-and-mouth disease virus, in particular to O-type foot-and-mouth disease virus variant strain, the gene and encoded amino acid sequence of the O-type foot-and-mouth disease virus variant strain, and the dominant neutralizing epitope of the amino acid sequence, and the present invention also relates to the gene and amino acid sequence The use of the dominant neutralizing epitope in the preparation of a vaccine for preventing or treating O-type foot-and-mouth disease virus belongs to the field of prevention and treatment of O-type foot-and-mouth disease virus. Background technique [0002] Due to the infidelity and high-speed replication of RNA-dependent RNA polymerase, RNA viruses have a high degree of genetic variation. More importantly, this genetically diverse progeny virus can survive a variety of selection pressures such as host immunity and antiviral therapy, leading to the virus's resurgence. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/42C12N7/00C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10C07K14/09C07K7/08A61K48/00A61K39/135A61P31/14
Inventor 于力杨德成于永忠周国辉
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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