American ginseng saponin F6 as well as extraction method and medical application thereof
A technology of American ginseng saponin and extraction method, which is applied in the field of American ginseng saponin F6 and its extraction and its medical application, and can solve problems such as not seen
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Embodiment 1
[0094] A. Extraction: Take 10kg of fresh American ginseng fruit, crush and filter to obtain American ginseng juice, pass D 101 The macroporous resin column was washed with water until it was colorless, and then eluted with 95% ethanol, and the ethanol eluate was collected, and the ethanol was recovered under reduced pressure, until dry, and vacuum-dried to obtain 180 g of total saponins of American ginseng fruit.
[0095] B. Separation: Take 100 g of total saponins of American ginseng fruit and perform silica gel column chromatography on 200 meshes, elute with chloroform-methanol 1:1, collect part of the eluate of chloroform-methanol 1:10, recover the solvent to dryness, and then pass through ODS Column chromatography, eluted with 68% methanol, American ginsenoside F 6 Partially recover the solvent to dryness to obtain American ginsenoside F 6 Crude product, about 0.02% yield.
[0096] C. American ginsenoside F 6 Purification: take crude American ginsenoside F 6 Further pa...
Embodiment 2
[0099] A. Extraction: Take 10kg of fresh American ginseng fruit, crush and filter to obtain American ginseng juice, pass D 4020 The macroporous resin column was washed with water until it was colorless, and eluted with 95% ethanol instead, the ethanol eluate was collected, the ethanol was recovered under reduced pressure, until dry, and vacuum-dried to obtain 165 g of total saponins of American ginseng fruit.
[0100] B. Separation: Take 100 g of total saponins of Panax ginseng fruit and carry out silica gel column chromatography with 300 meshes, elute with chloroform-methanol 1:5, collect part of the eluate of chloroform-methanol 1:10, recover the solvent to dryness, and then pass through ODS Column chromatography, eluted with 68% methanol, American ginsenoside F 6 Partially recover the solvent to dryness to obtain American ginsenoside F 6 Crude product, about 0.021% yield.
[0101] C. American ginsenoside F 6 Purification: take crude American ginsenoside F 6 Further part...
Embodiment 3
[0104] A. Extraction: Take 10kg of fresh American ginseng fruit, pulverize and filter to obtain American ginseng juice, pass through an AB8 macroporous resin column, wash with water until colorless, use 95% ethanol to elute, collect ethanol eluate, reclaim ethanol under reduced pressure, and dry , and vacuum-dried to obtain 170 g of total saponins of American ginseng fruit.
[0105] B. Separation: Take 100 g of total saponins of American ginseng fruit and perform silica gel column chromatography 300 mesh, elute with chloroform-methanol 20:1, collect part of the eluate of chloroform-methanol 1:10, recover the solvent to dryness, and then pass through ODS Column chromatography, eluted with 68% methanol, American ginsenoside F 6 Partially recover the solvent to dryness to obtain American ginsenoside F 6 Crude product, about 0.02% yield.
[0106] C. American ginsenoside F 6 Purification: take crude American ginsenoside F 6 Further partial recrystallization with ethanol-water t...
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