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Tissue culture method for tulbaghia violacea

A technology of tissue culture and Zijiaohua, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow planting of ramets or bulbs, limited seedlings, and a small number of Zijiaohua introductions, and achieves improvement. Propagation speed and uniformity of seedlings, and the effect of improving stability

Inactive Publication Date: 2012-01-04
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a new variety introduced from abroad, the number of introduced species of Zijiaohua is relatively small, and the planting of its ramets or bulbs is slow, so the supply of seedlings is limited to a certain extent and cannot meet the needs of the current market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Obtaining sterile materials

[0029] Take the germinated young buds and flower buds in spring, remove the leaves, wash them with tap water for 1 hour, put them on an ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and then soak them in mercury with a volume concentration of 0.5‰ for 10 minutes. Rinse 4 times with sterile water, use sterile filter paper to blot the water on the surface, then cut into 0.5cm-long segments and buds with axillary buds, and inoculate the segments and buds in a medium containing MS+6-BA2.0mg / On the bud induction medium of L+NAA0.2mg / L;

[0030] (2) Differentiation and proliferation of buds

[0031] After the segments and buds were inoculated on the bud induction medium for 2 weeks, the axillary buds began to expand, and yellow-green protrusions appeared. After 4 weeks, bud meristems could be seen. After culturing for 1 month, small adventitious buds could grow to 2 cm. Cut off the adventitiou...

Embodiment 2

[0040] (1) Obtaining sterile materials

[0041] Take the germinated young buds and flower buds in spring, remove the leaves, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 30 seconds, and then soak them in mercury with a volume concentration of 1‰ for 15 minutes. Rinse 5 times with sterile water, use sterile filter paper to blot the water on the surface, then cut into 1cm-long segments and buds with axillary buds, and inoculate the segments and buds in a medium containing MS+6-BA5.0mg / L +NAA0.5mg / L bud induction medium;

[0042] (2) Differentiation and proliferation of buds

[0043] After the segments and buds were inoculated on the bud induction medium for 3 weeks, the axillary buds began to swell, and yellow-green protrusions appeared. After 5 weeks, bud meristems could be seen. After 2 months of further cultivation, small adventitious buds could grow to 3 cm. The adventitious buds were...

Embodiment 3

[0052] (1) Obtaining sterile materials

[0053] Take the germinated young buds and flower buds in spring, remove the leaves, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and then soak them in mercury with a volume concentration of 2‰ for 30 minutes. Rinse 6 times with sterile water, use sterile filter paper to blot the water on the surface, then cut into 2cm-long segments and buds with axillary buds, and inoculate the segments and buds with MS+6-BA5.0mg / L +NAA0.5mg / L bud induction medium;

[0054](2) Differentiation and proliferation of buds

[0055] After the segments and buds were inoculated on the bud induction medium for 4 weeks, the axillary buds began to expand, and yellow-green protrusions appeared. After 6 weeks, bud meristems could be seen. After 2 months of cultivation, small adventitious buds could grow to 3 cm. The adventitious buds were cut and transferred to th...

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Abstract

The invention relates to a tissue culture method for tulbaghia violacea. The method comprises the following steps of: obtaining sterile materials; differentiating and proliferating buds; culturing strong seedlings of adventitious buds; rooting; and hardening off seedlings and transplanting the seedlings and the like. Compared with the prior art, the method greatly improves the reproductive speed of the tulbaghia violacea and the trimming of the seedlings, and improves the stability of shape and properties of the tulbaghia violacea, so that industrial mass production of the seedlings can be realized.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of Zijiao flower. Background technique [0002] Zijiaohua is a plant of the genus Allium in the Liliaceae family. Its origin is South Africa. It is a bulbous flower with a plant height of 30-50cm. It has small cylindrical bulbs and grows in clusters. The stems and leaves all contain leek flavor, and there are cyme-like inflorescences on the top, with small purple-pink flowers and a long flowering period. It is a good material for garden planting, potted planting or cut flowers. However, as a new variety introduced from abroad, the number of introduced species of Zijiaohua is relatively small, and the planting of its ramets or bulbs is slow, so the supply of seedlings is limited to a certain extent and cannot meet the current market demand. Contents of the invention [0003] The object of the present invention is exactly to provide a kind of tissue cultur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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