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Method for cellular tissue multiplication from jatropha curcas

A Jatropha curcas, cell technology, applied in plant cells, tissue culture, botanical equipment and methods, etc., can solve problems such as limitations

Inactive Publication Date: 2010-10-27
EMPRESAS PUBLICAS DE MEDELLIN E S P +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Unfortunately, the possibility to produce oil from Jatropha curcas seeds in large yields for biodiesel use is limited due to the huge land area required to harvest the plants produced by micropropagation or conventional propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0142] Ripe Jatropha curcas seeds were pre-cleaned and washed with water and soap, the seeds were sterilized using an ethanol bath; the seeds were then hydrated by wrapping them in paper moistened with sterile water; subsequently , using a 3% sodium hypochlorite bath to sterilize the seeds; thereafter, removing the skin of the seeds with a knife; cutting 1 to 2 mm thick explants or cross-cut layered parts from the cotyledons of the Jatropha curcas seeds obtained.

[0143] The explant of the Jatropha curcas seed cotyledon of 1 to 3 grams is placed in 100 milliliters of culture mediums with following composition and concentration:

[0144] NH 4 NO 3 400mg / L

[0145] CaNO 3 383mg / L

[0146]CuSO 4 0.25mg / L

[0147] MnSO 4 22.3mg / L

[0148] ZnSO 4 8.6mg / L

[0149] h 3 BO 3 6.2mg / L

[0150] K H 2 PO 4 170...

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PUM

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Abstract

The method of the present invention comprises: obtaining an explant from the seeds of Jatropha curcas; putting the explant derived from the seed of Jatropha curcas in a culture medium; breaking the intercellular unions of the explants tissue, which generates individuals cells; incubating for a determined time the culture medium with the generated individual cells, that were multiplied; and, extracting oil from the cells that multiplied from the individual cells generated from the explants derived from the Jatropha curcas seed.

Description

technical field [0001] The present invention relates to the expansion of cells derived from oleaginous plant seeds, and to obtaining oil from the cells produced. Background technique [0002] The seeds of oleaginous plants have been identified as a source for the production of oils that can be used for uses such as biodiesel. [0003] An example is the Jatropha curcas plant, which has oleaginous seeds. In addition to its use in medicine, veterinary medicine and soap making, etc., the oil is considered suitable as high-quality biodiesel. Oil derived from Jatropha curcas seeds has similar physicochemical properties and output characteristics to diesel fuel used in engines (see Murali et al., US Patent Application Publication No. 2008 / 0194026A1, paragraphs 0008-0010). [0004] Thus, in the prior art, micropropagation methods for producing Jatropha plants from organs, tissues, cells or protoplasts have been described. (See Murali et al., paragraphs 0014, 0017 and 0024). The ...

Claims

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Application Information

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IPC IPC(8): C10L1/02C12N5/04C12P7/649
CPCY02E50/13C12P7/649C12P7/6463A01H4/00Y02P30/20Y02E50/10C12N5/00C12N5/04C12P7/64
Inventor 露西娅·阿特霍尔图亚加尔塞斯桑德拉·马塞拉·科雷亚科尔多巴
Owner EMPRESAS PUBLICAS DE MEDELLIN E S P