Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Electroporated chip and porous plate device base on electroporated chip

An electroporation, multi-well plate technology, applied in the method of supporting/immobilizing microorganisms, the method of stress-stimulating the growth of microorganisms, biochemical equipment and methods, etc., can solve problems such as reducing work efficiency, and achieve the effect of reducing equipment costs

Active Publication Date: 2010-10-27
ETTA BIOTECH
View PDF6 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In the disclosed prior art, cell culture and electroporation are performed separately, which brings extra workload and reduces work efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electroporated chip and porous plate device base on electroporated chip
  • Electroporated chip and porous plate device base on electroporated chip
  • Electroporated chip and porous plate device base on electroporated chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0058] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0059] 1 Substrate:

[0060] Because the substrate acts as a support and acts as insulation and isolation between electrodes, the substrate needs to be made of insulating material, or supported by a non-insulating material covered with an insulating layer. The substrate according to the present invention can be made from any solid substrate suitable for the above conditions, preferably those substrates which can be molded or machine cut to the required specifications. Transparent glass or polymer is particularly preferred, since in this case the state of the cells can be observed in real time through a microscope. Such as figure 1 As shown, the porous plate device used for electroporation includes: a porous plate 1; holes 2 and 3 of the porous plate; a substrate 4; an electroporation chip array 5 below the porous plate (the specific arrangemen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
widthaaaaaaaaaa
heightaaaaaaaaaa
Login to View More

Abstract

The invention discloses an electroporated chip and a porous plate device for electroporation. The porous plate device comprises a plurality of electroporated chips, a porous plate, a voltage source and an electrical connecting part, wherein the electroporated chip comprises a baseplate and electrodes, the baseplate is used for bearing the electrodes, every two electrodes are used as a pair, each electrode comprises an anode and a cathode which are oppositely arranged, and the anode and the cathode are mutually nested; the porous plate comprises a plurality of holes, the porous plate is placedon the baseplate of each electroporated chip to form a cavity body, and the bottom of each hole corresponds to one electroporated chip; the voltage source is used for setting and generating pulse voltages; and the electrical connecting part is used for connecting the voltage source and the electroporated chips. The device of the invention has higher electroporation effect and can realize large-batch high-efficiency sample processing.

Description

technical field [0001] The present invention relates to an electroporation chip, and more specifically, the present invention relates to a porous plate device for efficiently utilizing the electroporation chip to perform electroporation. Background technique [0002] The cell membrane is a layer of thin film surrounding the periphery of the cell, which is a permeability barrier for the selective material exchange between the cell and the outside world. The cell membrane makes the cell an independent living unit and has a relatively stable internal environment. Some substances in the surrounding environment can pass through the cell membrane, while others cannot. Cells can absorb nutrients from the surrounding environment through the cell membrane, discharge metabolites, and make the transport of substances reach a state of balance. Therefore, the basic function of the cell membrane is to maintain the relative stability of the intracellular microenvironment and selectively ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42
CPCC12M25/08C12M35/02
Inventor 梁子才李志宏杜权黄璜魏泽文
Owner ETTA BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com