Method for preventing and treating protozoa in chlorella culture solution

A technology of protozoa and culture solution, which is applied in the field of prevention and control of protozoa in green algae culture solution, can solve the problems of ineffective stabilization of chlorella culture, slow growth and reproduction of algae, and sinking of algae cells, and achieve drug residue time Short, fast treatment, low cost effect

Inactive Publication Date: 2012-05-23
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

However, practice has proved that in large-scale industrial production, the effect of the above method is not ideal, often resulting in slow growth and reproduction of algae, causing a large number of algae cells to sink, and even causing algae to die, so the cultivation of Chlorella cannot be effectively stabilized

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Embodiment Construction

[0006] The concrete implementation method of the present invention is as follows:

[0007] Disinfect algae cultivation tools: Take industrial hydrochloric acid and fresh water to make a 0.1mol / L hydrochloric acid solution, put the cleaned air stones and trachea and other non-metallic cultivation tools into the hydrochloric acid solution for 10 minutes, then rinse with sterilized seawater for 2~ 3 times.

[0008] The culture pool adopts tile pool with a volume of 1-10t; pour the sand-filtered seawater into the clean culture pool, accurately calculate the effective water body in the culture pool, and use sodium hypochlorite containing 8% available chlorine at a concentration of 250mg / L to treat the culture pool and Disinfect the seawater together to eliminate protozoa. After disinfection for 12-24 hours, use sodium thiosulfate at a concentration of 20mg / L to neutralize residual chlorine, and then add chlorella to the culture medium according to the formula of the culture medium....

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Abstract

The invention provides a method for preventing and treating protozoa in a chlorella culture solution, which relates to prevention and treatment of protozoa in a chlorella culture solution. In the method, tools, a culture tank and sea water for cultivating alga are disinfected. The method is characterized in that soaking cleaned air stones and an air pipe into a hydrochloric acid solution of 0.1mol / L for 10min, and flushing the air stones and the air pipe with the disinfected sea water 2-3 times; filling sea water filtered in the culture tank; disinfecting the culture tank and the sea water together with sodium hypochlorite containing 8% of active chlorine in a concentration of 250mg / L; after reducing with sodium thiosulfate, introducing alga seeds immediately after adding nutrient substances in the culture solution for culturing the alga seeds; after introducing the alga seeds, adding formaldehyde to the culture solution in a concentration of 10mg / L; and repeating the procedures for two days, one time for each day, to prevent the generation of the protozoa. The method is used for preventing teh protozoa in the chlorella culture solution.

Description

technical field [0001] The invention relates to a method for preventing and controlling protozoa in a green algae culture solution. Background technique [0002] Chlorella (Chlorella) belongs to the single-celled algae of the genus Chlorella in the phylum Chlorella, and there are more than a dozen common species such as Chlorella pyrenoidosa, Chlorella marine, Chlorella eyepoint, and Chlorella ovale. Chlorella has been valued due to its high light energy efficiency, rich nutrition, and rapid growth and reproduction. The application of chlorella mainly includes the following aspects: as health food, food and feed additives, biologically active substances and sewage treatment substances . In the field of aquaculture, chlorella has become one of the main biological baits that are mass-cultivated and widely used in the production of rotifers and aquatic animal seedlings. However, during the cultivation process, protozoa often contaminate Chlorella. The common protozoa include...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12A61L2/18C02F1/50A01N35/02A01P1/00C12R1/89A61L101/06
Inventor 陆建学夏连军来琦芳王慧
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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