Light chain and heavy chain variable region of FMU-EPCAM-2D7 monoclonal antibody
A technology of FMU-EPCAM-2D7, 1. FMU-EPCAM-2D7, applied in the field of monoclonal antibodies, can solve problems such as antibody drug hypersensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0030] The applicant immunized BALB / c mice with recombinant human EPCAM, prepared a group of mouse anti-human EPCAM monoclonal antibodies, and screened out the FMU-EPCAM-2D7 monoclonal antibody hybridoma cell line that can stably secrete high-affinity anti-human EPCAM, Ascites fluid was prepared to obtain high-affinity anti-human EPCAM monoclonal antibody. Confirm the uniqueness of the gene sequence and corresponding protein sequence and its CDR sequence; provide support for anti-human EPCAM chimeric or humanized genetic engineering antibodies.
[0031] The present invention will be described in detail below in conjunction with the accompanying drawings, which are explanations of the present invention rather than limitations.
[0032] The present invention is specifically implemented according to the following steps:
[0033] 1 Preparation of mouse anti-human EPCAM high-affinity antibody
[0034] 1.1 Preparation and purification of monoclonal antibodies
[0035] Design prim...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com