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Application of CAF22 detection reagent in preparation of composition for evaluating renal tubular injury

A kidney tubule and composition technology, applied in the field of biological diagnosis, can solve the problems of poor diagnostic specificity of renal tubule injury, loss of treatment time, and inability to confirm abnormal renal function, etc., and achieve the effect of multi-possibility, improved sensitivity and accuracy

Active Publication Date: 2022-06-03
BEIJING WATSON-CESAR BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Second, Scr is a surrogate indicator of glomerular filtration rate (GFP), and its concentration indicates the balance of creatinine production and excretion, but it will be reflected in the increase of SCr when GFP is lower than 50% of the normal level, so Scr will delay the diagnosis of renal tubular injury; third, SCr will be affected by many external factors such as the patient's physiology, pathological state and drug use
Fourth, changes in urine output may not be consistent with the degree of renal injury. For example, dehydration or use of diuretics will lead to changes in urine output, and the specificity of urine output for the diagnosis of renal tubular injury is poor
However, the specific renal state cannot be reflected, and the specific cause of abnormal renal function cannot be confirmed. In clinical practice, the best treatment time may be lost due to the inability to correctly judge the state of renal injury, and early intervention of renal injury will lead to Reducing mortality and shortening the duration of treatment for all types of tubular injury, including but not limited to ischemic and nephrotoxic kidney injury, associated with renal tubular injury

Method used

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  • Application of CAF22 detection reagent in preparation of composition for evaluating renal tubular injury
  • Application of CAF22 detection reagent in preparation of composition for evaluating renal tubular injury
  • Application of CAF22 detection reagent in preparation of composition for evaluating renal tubular injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Sample Collection

A total of 157 urine samples with abnormal renal status were collected.

[0035] The abnormal renal status is manifested as: abnormal albumin, immunoglobulin G, transferrin, α1 microglobulin, lysosomal enzymes, and creatinine indexes in urine.

Embodiment 2

[0036] Embodiment 2, the preparation method of CAF22 chemiluminescence detection kit

The CAF22 chemiluminescence detection kit of the present invention mainly includes a conjugate-labeled CAF22 antibody, a chemiluminescence label-labeled CAF22 antibody, streptavidin magnetic particles, a chemiluminescence substrate, a CAF22 standard and a quality control product.

[0037] The preparation method of the CAF22 chemiluminescence detection kit of the present invention mainly comprises the following steps:

Step 1) Preparation of conjugate-labeled CAF22 antibody

The activator biotin ester was added to the CAF22 antibody after desalting in a molar ratio of 1:10, and the reaction was performed at room temperature for 4 hours in the dark. The reacted solution was added to the pre-rinsed PD-10 purification column for purification. The purified conjugate was measured with A280 and stored at -20°C after adding an equal volume of glycerol. When in use, dilute to a concentration of 0.1...

Embodiment 4

[0046] Example 4. Method for detecting CAF22 with CAF22 chemiluminescence detection kit

The chemiluminescence detection kit for determining CAF22 of the present invention includes reagent R1, reagent R2, streptavidin magnetic beads, chemiluminescence substrate, CAF22 calibrator and quality control substance.

[0047] R1 is a biotin-conjugated CAF22 antibody, stored in 50 mM PBS pH 7.4 and 0.15 M NaCl, the buffer also contains 0.1% sodium azide, 0.5 g / L Tween 20 and 1 g / L bovine serum albumin.

[0048]R2 is an alkaline phosphatase-labeled antibody to CAF22, maintained in 0.05 M PBS and 0.15 M NaCl in a buffer also containing 0.1% sodium azide, 0.5 g / L Tween 20, and 1 g / L sodium caseinate.

[0049] The concentration of streptavidin magnetic beads is 1 mg / mL, and the particle size of the magnetic beads is 2.8 μM.

[0050] The chemiluminescent substrate is APS-5.

[0051] A fully automatic chemiluminescence immunoassay was used as the detection tool, the detection mode was san...

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Abstract

The invention discloses application of a compact protein C-terminal fragment (CAF22) detection reagent in preparation of a renal tubule injury diagnostic kit and the renal tubule injury diagnostic kit, and provides a new biomarker for diagnosis of renal tubule injury by using the CAF22 as a diagnostic marker of the renal tubule injury. According to the invention, CAF22 detection is combined with other renal injury biomarkers, so that the accuracy and sensitivity of early diagnosis of renal tubule injury can be improved, and the kit has high application value in early diagnosis and treatment of renal injury.

Description

technical field [0001] The invention relates to the field of biological diagnosis, in particular to the application of a reagent for detecting CAF22 in the preparation of a composition for evaluating renal tubular damage. Background technique [0002] Acute Kidney Injury (Acute Kidney Injury) is a common complication in critically ill patients and cardiovascular surgery patients. It has high morbidity and mortality. The incidence rate is about 5%-47% after cardiac surgery, and ICU patients. The mortality rate of renal tubular injury is as high as 50%-70%. If left untreated, renal tubular damage may progress to chronic kidney disease or require permanent dialysis. Therefore, early diagnosis and timely intervention and treatment of renal tubular injury can effectively prevent further deterioration of the disease and improve the prognosis of patients. In order to improve the diagnosis and treatment of renal tubular injury and improve the global prognosis of renal disease (KDI...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N33/6869G01N2800/60G01N2333/47G01N2800/50G01N2800/52G01N2800/56G01N2800/347
Inventor 唐明镜郑旻亮席强
Owner BEIJING WATSON-CESAR BIOTECHNOLOGY CO LTD
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