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Bacillus subtilis GU and application in preparing efficient biological compound phosphate fertilizer thereof

The technology of Bacillus subtilis and compound phosphate fertilizer is applied in the field of Bacillus subtilis in soil and high-efficiency biological compound phosphate fertilizer. Ability to increase, remove the effect of spots

Inactive Publication Date: 2010-12-01
辛伟成
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the phosphorus utilization rate of phosphorus fertilizers commonly used in agriculture is generally not high. The existing biological fertilizers are not only expensive, but also slow to take effect, and lose their vitality quickly.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment one: the production of bacterial powder

[0042] 1. Inoculate into liquid seed medium from a slant, and incubate at 37°C for 24 hours.

[0043] The solid slant medium is: 0.6% peptone, 0.3% beef extract, 1.8% agar, pH 6.8, and sterilized at 121° C. for 20 minutes.

[0044] The liquid seed medium is: peptone 0.8%, beef extract 0.3%, yeast extract 0.1%, NaCl 0.5%, KCl 0.2%, MgSO 4 ·7H 2 O 0.05%, CaCl 2 0.002%, MnSO 4 ·H 2 O 0.001% g, pH 6.8, sterilized at 121°C for 20min.

[0045] 2. After the seed culture is completed, put it into the fermenter with an inoculation amount of 0.2%.

[0046] The fermentation medium is: 1.1% corn starch, 3% corn steep liquor dry powder, 0.1% yeast extract, 0.2% peptone, KH 2 PO 4 0.2%, Na 2 HPO 4 0.5%, MgSO 4 ·7H 2 O 0.02%, CaCl 4 0.02%, MnSO 4 ·H 2 O0.005% g, pH 6.8.

[0047] The fermentation culture conditions are as follows: culture at 37° C. for 50 hours, rotation speed 250 rpm, dissolved oxygen content kep...

Embodiment 2

[0052] Embodiment two: the production of biological compound phosphate fertilizer

[0053] 1. Select organic carrier bran, rice husk powder, and peanut husk powder in a ratio of 2:3:1, add 3% bacterial powder by mass ratio, and mix thoroughly.

[0054] 2. Mix the bacteria powder mixed with the carrier with phosphate rock powder at a ratio of 1:7, and add the strengthening stimulating factor.

[0055] 3. The ingredients of strengthening stimulating factors are: 0.16% manganese sulfate, 0.18% copper sulfate, 1.4% urea, 1.5% ammonium citrate, and 0.15% peptone.

[0056] 4. Granulate after mixing.

[0057] 5. Store in a cool and dry place after fully mixing.

[0058] 6. The number of live bacteria of this biological compound phosphate fertilizer is 1.2×10 8 , the total phosphorus content is 11.2%, and the water-soluble phosphorus content is 0.6%.

Embodiment 3

[0059] Embodiment three: application test

[0060] Experimental crop: Banana

[0061] Test sample: In the experiment, two other phosphate fertilizers were used as controls, and clear water was used as blank control.

[0062] Soil Type: Brick Red Soil

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PUM

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Abstract

The invention discloses bacillus subtilis GU and application in preparing efficient biological compound phosphate fertilizer thereof. The culture preservation number of the bacillus subtilis GU is CCTCC No: M209228, and the bacillus subtilis GU has an efficient phosphate-dissolving ability and can be used for preparing the efficient biological compound phosphate fertilizer. The invention also discloses the efficient biological compound phosphate fertilizer which contains organic carriers, phosphorin raw materials and reinforced stimulus factors, and the organic carriers contain powder of the bacillus subtilis GU. In the efficient biological compound phosphate fertilizer, the powder of the bacillus subtilis GU, powdered rock phosphate and the reinforced stimulus factors are mixed in proportion, the efficient biological compound phosphate fertilizer has strong phosphorus supply ability, the content of dissolvable phosphorus in soil is greater than 25mg / kg for 6 months after primary fertilization, and the fertilizer has significance for increasing production of crop farming in China.

Description

technical field [0001] The invention relates to a soil phosphate bacillus that can be used for producing high-efficiency biological compound phosphate fertilizer and a high-efficiency biological compound phosphate fertilizer produced by the strain. technical background [0002] Phosphorus is one of the essential mineral elements for plant growth, and is an important component of nucleic acid in plants, and nucleic acid is an important component of nucleoprotein formation. Nucleic acid is widely involved in the composition of protoplasm and organelles. More than 95% of the phosphorus in the soil is in an invalid form, mainly forming mineral phosphate compounds with some metal ions, which are difficult for plants to directly absorb and utilize. The utilization rate of chemical fertilizers and phosphorus fertilizers in current crops is only 5% to 25%. There is a large area of ​​phosphorus-deficient soil in my country, and 74% of the arable land is deficient in phosphorus. Even ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/125
Inventor 曹昱
Owner 辛伟成
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