Multi-mode imaging system for observing cerebral cortex functions of moving animals

Abstract

The invention discloses a multi-mode imaging system for observing cerebral cortex functions of moving animals, comprising a light source device, an image transferring optical fiber, a light transferring optical fiber, a spectroscope, an intrinsic signal imaging part, a laser speckle contrast imaging part, a fluorescence Imaging part, an animal activity room, an image acquisition card, a computer, wherein the light source device generates three light sources needed by an imaging mode, the image transferring optical fiber is used for transferring the cerebral cortex images to a spectroscope when the animals moves around freely; the light transferring optical fiber transfers the light of the light source to the cerebral cortex of the aminals; the spectroscope divides imaging light beam transferred by the image transferring optical fiber into three beams and carries out intrinsic signal imaging, laser speckle contrast imaging and fluorescence Imaging; the intrinsic signal imaging part is used for the intrinsic signal imaging; the laser speckle contrast imaging part for laser speckle contrast imaging; the fluorescence Imaging part is used for fluorescence Imaging; the animal activity room is used for the experiment animals to move freely; the image acquisition card is used for image acquisition and the computer for system control and image receiving and processing.In the invention, optical fiber light and image transferring can be used to multi-mode imaging when the animals are conscious and active and the invention comprises intrinsic signal imaging, laser speckle contrast imaging and fluorescence Imaging.

Description

technical field [0001] The present invention relates to biomedical imaging technology, especially the combination of endogenous signal optical imaging technology, laser speckle contrast imaging technology and fluorescence imaging technology. pattern imaging. Background technique [0002] Brain function optical imaging has always been an extremely important research method in the field of neuroscience research. At present, the optical imaging methods of brain function widely carried out at home and abroad include various imaging methods such as fluorescence imaging, optical imaging of endogenous signals, near-infrared light imaging, and laser speckle imaging. Most of these imaging methods are applied to small animals that have been anesthetized and fixed on the bench. Although optical imaging systems based on anesthetized small animals have been widely used, and can provide a very stable platform for scientific research on brain function, the physiological responses of ani...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a multi-mode imaging system that can be used for the observation of cerebral cortex function of awake animals. Contrast Imaging and Fluorescence Imaging Issues

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