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Device and method for preparing hollow heteromorphic bacteria cellulose material

A bacterial cellulose and special-shaped technology, which is applied in the field of devices for preparing hollow special-shaped bacterial cellulose materials, can solve problems such as inability to carry out industrial production and application, rough outer or inner surfaces of materials, unsatisfactory production costs and production efficiency, etc. , to achieve good application prospects, simple and easy preparation method, high strength effect

Active Publication Date: 2010-12-22
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the production cost and production efficiency of the prior art methods are unsatisfactory, and the outer surface or inner surface of the material is rough, so it cannot be industrialized production and application

Method used

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  • Device and method for preparing hollow heteromorphic bacteria cellulose material
  • Device and method for preparing hollow heteromorphic bacteria cellulose material
  • Device and method for preparing hollow heteromorphic bacteria cellulose material

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0056] like figure 1 , figure 2 , image 3 , Figure 4 As shown, a device for preparing hollow special-shaped bacterial cellulose material, a closed container 1, a second tubular part 2, a first tubular part 4 and a cylindrical hollow plug 5, the first tubular part 4 and the second tubular part 2 are coaxially sleeved In the lumen of the second tubular part 2, the opening of the second tubular part 2 is sealed with the first tubular part 4 by a cylindrical hollow plug 5, and the gap between the first tubular part 4 and the second tubular part 2 is poured into the fermentation medium space 3.

[0057] The first tubular part 4 and the second tubular part 2 are cylindrical, or multi-branched, or hand-shaped and hollow. The cross-sectional shapes of the second tubular member 2 and the first tubular member 4 are circular, square, elliptical, triangular, heart-shaped, and pentagram-shaped. The space 3 for pouring the fermentation medium, the second tubular part 2 and the cylin...

Embodiment 2

[0061] (1) Bacteria culture

[0062] Acetobacter xylinum (Acetobacter xylinum) ATCC23770 was inserted into the liquid seed medium according to the inoculation amount of 2 to 3 inoculation loops, cultivated on a shaker at 30°C and 160r / min for 12 hours, and then set aside;

[0063] Among them, the components of the seed medium are: 25g of mannitol, glucose, maltose, sucrose or fructose, 3g of peptone or tryptone, 5g of yeast extract, 1L of water, pH5.0, sterilized at 121°C for 20min;

[0064] (2) Fermentation preparation of tubular BC material

[0065] Two silicone tube materials with different outer diameters, thicknesses and lengths but with gas permeability are fixed together axisymmetrically. One of the outer tubes has an outer diameter of 9 mm, a thickness of 0.5 mm, and a length of 6 cm (the second Tubular part), another inner tube has an outer diameter of 3 mm, a thickness of 0.5 mm, and a length of 8 cm (the first tubular part, such as figure 1device), inject 1.5mL of...

Embodiment 3

[0069] (1) Bacteria culture

[0070] Kombucha is inserted into the liquid seed medium according to the inoculum amount of 1-10 discs with a diameter of 0.5 cm, and cultured on a shaking table at 30 ° C and 160 r / min or standing for 12-24 hours; then press Transfer the inoculum of 1-10 discs with a diameter of 0.5-1em containing bacteria BC membrane to the liquid fermentation medium, and culture it on a shaking table at 30°C and 160r / min or stand it for 12-24 hours before use;

[0071] Among them, the preparation of liquid seed medium and fermentation medium: boil 1L of water, add 1-10g of green tea or black tea (5g of tea leaves is the best), soak for 20 minutes, filter out the tea leaves to obtain tea soup, and then add glucose, sucrose or fructose 25-100g, peptone or tryptone 3g, yeast extract 5g, pH5.0-6.0, pasteurized for 30min;

[0072] (2) Fermentation preparation of tubular BC material

[0073] Two tubular materials with different outer diameters, thicknesses and leng...

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Abstract

The invention relates to a device and method for preparing a hollow heteromorphic bacteria cellulose material. The device comprises an airtight container, a second tubular component, a first tubular component and a cylindrical hollow plug, wherein the first tubular component and the second tubular component are coaxially sheathed inside the lumen of the second tubular component; an opening of the second tubular component is sealed with the first tubular component by the cylindrical hollow plug; and the gap between the first tubular component and the second tubular component is a space for storing fermentation medium. The method comprises the following steps: after inoculating fluid nutrient medium with bacterial cellulose-producing strain for spread cultivation, transferring the bacterial cellulose-producing strain to a fermentation device with an oxygen permeability mould, and culturing to obtain the heteromorphic bacteria cellulose material. The hollow heteromorphic bacteria cellulose (BC) material prepared by the invention not only has controllable size and shape, but also has the detachable and reused mould of the fermentation device, can be widely applied to substitute goods of hollow organs, such as artificial vascular grafts, nerve fiber conduits and the like, and can be served as the material for packing the foods, such as casings for meat products, jelly coatings and the like.

Description

technical field [0001] The invention belongs to the field of preparation of bacterial cellulose materials, in particular to a device and method for preparing hollow special-shaped bacterial cellulose materials. Background technique [0002] Bacterial Cellulose (BC) is widely used in artificial blood vessels, Tissue engineering scaffolds, artificial skin, and treatment of skin injuries have a wide range of uses, and are one of the hotspots in international biomedical material research. [0003] As early as the beginning of the last century, autologous blood vessel transplantation has been applied to arterial vessel reconstruction, but the source of autologous blood vessels is limited. Fused into the patient's tissue and kept in close contact, so that some clinical problems such as implant loosening, tissue damage near the implant, pain, and surgical revision can be avoided. Especially in the materials for transplantation, artificial blood vessel materials are in direct cont...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/04C12P19/04C12R1/02C12R1/01C12R1/41C12R1/38C12R1/025C12R1/05C12R1/065
CPCC12M23/24C12M23/34
Inventor 洪枫魏斌杨光
Owner DONGHUA UNIV
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