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Influenza A virus medicament screening yeast two-hybrid system and application thereof

A two-hybrid technology of influenza A virus and yeast, which is applied in the field of genetic engineering, can solve the problems of screening specificity, low screening throughput, unclear mechanism of action of target drugs, etc., and achieve high specificity effect

Inactive Publication Date: 2013-07-31
SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the embodiment of the present invention is to provide a target-specific influenza A virus drug screening yeast two-hybrid system, to solve the existing technology for influenza A virus drug screening specificity, low screening throughput, unclear mechanism of action of the target drug and other technologies question

Method used

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  • Influenza A virus medicament screening yeast two-hybrid system and application thereof
  • Influenza A virus medicament screening yeast two-hybrid system and application thereof
  • Influenza A virus medicament screening yeast two-hybrid system and application thereof

Examples

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Effect test

Embodiment 1

[0033] Example 1 of the present invention provides a target-specific influenza A virus drug screening yeast two-hybrid system, using yeast two-hybrid plasmids pGADT7 and pGBKT7, the target-specific influenza A virus drug screening yeast two-hybrid system also includes The PB1 gene and the PB2 gene of the international standard strain of influenza A virus A / PR8 / 34, the PB1 gene is recombined with pGBKT7, and the PB2 gene is recombined with pGADT7.

[0034]The DNA sequence complementary to the PB1 gene is shown in SEQ ID NO: 1. In SEQ ID NO: 1, the base sequence from position 25 to position 2298 corresponds to PB1 of the international standard strain of influenza A virus A / PR8 / 34 The protein coding region of the gene; the DNA sequence complementary to the base sequence of the PB2 gene is shown in SEQ ID NO: 2, and in SEQ ID NO: 2, the base sequence from the 28th to the 2307th corresponds to the influenza A virus international The protein coding region of the PB2 gene of the stan...

Embodiment 2

[0038] Example 2 of the present invention provides a target-specific influenza A virus drug screening yeast two-hybrid system, using yeast two-hybrid plasmids pGADT7 and pGBKT7, the target-specific influenza A virus drug screening yeast two-hybrid system also includes The PB1 gene and PA gene of the international standard strain of influenza virus A / PR8 / 34, the PB1 gene is recombined with pGBKT7, and the PA gene is recombined with pGADT7.

[0039] The DNA sequence complementary to the base sequence of the PB1 gene is shown in SEQ ID NO: 1; the DNA sequence complementary to the base sequence of the PA gene is shown in SEQ ID NO: 3. In SEQ ID NO: 3, The 25th to 2175th base sequence corresponds to the protein coding region of the PA gene of the international standard strain of influenza A virus A / PR8 / 34

[0040] The exogenous gene sequence included in the target-specific influenza A virus drug screening yeast two-hybrid system is the gene sequence of PB1-pGBKT7 of the internation...

Embodiment 3

[0043] Example 3 of the present invention provides a target-specific influenza A virus drug screening yeast two-hybrid system, the target-specific influenza A virus drug screening yeast two-hybrid system includes the above-mentioned examples 1 and 2 target Yeast two-hybrid system for spot-specific influenza A virus drug screening.

[0044] Among them, the target-specific influenza A virus drug screening yeast two-hybrid system in Example 1 uses yeast two-hybrid plasmids pGADT7 and pGBKT7, and the target-specific influenza A virus drug screening yeast two-hybrid system also includes influenza A The PB1 gene and PB2 gene of the virus international standard strain A / PR8 / 34, the PB1 gene is recombined with pGBKT7, and the PB2 gene is recombined with pGADT7.

[0045] The target-specific influenza A virus drug screening yeast two-hybrid system of Example 2 uses yeast two-hybrid plasmids pGADT7 and pGBKT7, and the target-specific influenza A virus drug screening yeast two-hybrid syst...

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Abstract

The invention is suitable for the technical field of genetic engineering, and provides a target specific influenza A virus medicament screening yeast two-hybrid system and application thereof. The target specific influenza A virus medicament screening yeast two-hybrid system comprises an influenza A virus PB1gene and an influenza A virus gene, a PB1 gene and a PA gene, and PB1, PB2 and PA genes. The target specific influenza A virus medicament screening yeast two-hybrid system for medicament screening has the advantages of high specificity, high throughput screening and exact mechanism of a target medicament.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a target-specific influenza A virus drug screening yeast two-hybrid system and its application. Background technique [0002] Influenza viruses belong to the Orthomyxoviridae family (Orthomyxoviridae), which are negative-strand RNA viruses with envelopes. Members include influenza A, B, and C. Currently, influenza A and B mainly infect humans. The genomes of influenza A and B viruses contain 8 segments, which are single-strand and negative-strand RNAs, encoding at least 10 proteins respectively. Influenza A viruses can be divided into 1-16 HA subtypes and 1-9 NA subtypes according to the different antigenic properties of hemagglutinin (HA) and neuraminidase (NA) on the surface of the virus. Influenza viruses currently causing human infections are mainly H1 (N1), H3 (N2) subtypes and B influenza viruses. Influenza virus RNA-dependent RNA polymerase by PB 1 , PB 2 ,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/19C12N15/44C12Q1/02C12R1/85
Inventor 房师松何建凡张仁利
Owner SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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