Kit for human genotyping by fluorescence labeling STR and detection method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A genotyping and fluorescent labeling technology, which is applied in the direction of fluorescence/phosphorescence, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems that cannot meet the identification requirements, and achieve the effect of improving scientificity and reliability
Inactive Publication Date: 2010-12-22
HEBEI MEDICAL UNIVERSITY
View PDF1 Cites 7 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0003] However, for the single-parent identification of doublets or some other difficult genetic relationship identification, the above-menti
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0027] A fluorescent-labeled STR kit for human genotyping, which consists of a PCR amplification system and detection reagents; the PCR amplification system includes primers, PCR buffer solution, TaqDNA polymerase, template DNA, dNTP and MgCl 2 The detection reagents include deionized formamide solution and GS500LIZ internal standard; the primers are primers for the following loci: Amelogenin, D8S1132, D12S391, GATA198B05, D7S3048, D2S1772, D11S2368, D6S1043 and D13S325.
[0028] The components, concentrations and contents of the PCR amplification system are shown in Table 4.
[0029] The sequences of the primers are shown in Table 5.
[0030] The detection method of human genotyping using the above kit is carried out according to the following steps:
[0031] Step 1. Extract DNA and get DNA extract
[0032] Use clean ophthalmic scissors to cut out a blood spot about 3mm×3mm in size from the middle of the blood spot, and place it in a 1.5mL test tube (the ophthalmic scissors are soaked ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a kit for human genotyping by fluorescence labeling STR and a detection method thereof, belonging to the field of human gene analysis and detection.The kit comprises a) a PCR amplification system: primer, PCR buffer solution, TaqDNA polymerase, template DNA, dNPT and MgCL2, b) detection reagent: deionized formamide solution and a GS500LIZ interior label; the primer is the primer of the following gene loca: Amelogenin, D8S1132, D12S391, GATA198B05, D7S3048, D2S1772, D11S22368, D6S1043 and D13S325.The detection method is carried out in accordance with the following steps: extracting DNA, carrying out compound amplification on gene loca, detecting and determining amplification products.Jointly applied to single parent identification and difficult genetic relationship identification cases with the existing commercialized kits, the invention can enable identification result to be more scientific and act as the beneficial supplement of the existing STR kit.
Description
Technical field [0001] The invention relates to human gene detection, in particular to the analysis of STR (short tandem repeats, short tandem repeats), and specifically to a kit and a detection method for human genotyping using fluorescently labeled STR. Background technique [0002] The STR genetic marker system is widely used in forensic medicine, archaeology, genetics and other fields because it is widely distributed in the human genome, has a high degree of genetic polymorphism and simple and quick detection methods. At present, the commonly used STR commercial kits for personal identification and paternity testing mainly include AmpFLSTRIdentifiler, PowerPlex 16 two kinds. The AmpFLSTR Identifiler system includes 15 autosomal loci: D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, FGA, and a sex identification site Amelogenin; The 16 system includes 15 autosomal loci: Penta E, D18S51, D21S11, TH01, D3S1358, FGA,...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more