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Kit for human genotyping by fluorescence labeling STR and detection method thereof

A genotyping and fluorescent labeling technology, which is applied in the direction of fluorescence/phosphorescence, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems that cannot meet the identification requirements, and achieve the effect of improving scientificity and reliability

Inactive Publication Date: 2010-12-22
HEBEI MEDICAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0003] However, for the single-parent identification of doublets or some other difficult genetic relationship identification, the above-mentioned commercial kits are used alone, and the system efficiency of both is less than 0.9999, which cannot meet the identification requirements.

Method used

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  • Kit for human genotyping by fluorescence labeling STR and detection method thereof
  • Kit for human genotyping by fluorescence labeling STR and detection method thereof
  • Kit for human genotyping by fluorescence labeling STR and detection method thereof

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Embodiment Construction

[0027] A fluorescent-labeled STR kit for human genotyping, which consists of a PCR amplification system and detection reagents; the PCR amplification system includes primers, PCR buffer solution, TaqDNA polymerase, template DNA, dNTP and MgCl 2 The detection reagents include deionized formamide solution and GS500LIZ internal standard; the primers are primers for the following loci: Amelogenin, D8S1132, D12S391, GATA198B05, D7S3048, D2S1772, D11S2368, D6S1043 and D13S325.

[0028] The components, concentrations and contents of the PCR amplification system are shown in Table 4.

[0029] The sequences of the primers are shown in Table 5.

[0030] The detection method of human genotyping using the above kit is carried out according to the following steps:

[0031] Step 1. Extract DNA and get DNA extract

[0032] Use clean ophthalmic scissors to cut out a blood spot about 3mm×3mm in size from the middle of the blood spot, and place it in a 1.5mL test tube (the ophthalmic scissors are soaked ...

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Abstract

The invention discloses a kit for human genotyping by fluorescence labeling STR and a detection method thereof, belonging to the field of human gene analysis and detection.The kit comprises a) a PCR amplification system: primer, PCR buffer solution, TaqDNA polymerase, template DNA, dNPT and MgCL2, b) detection reagent: deionized formamide solution and a GS500LIZ interior label; the primer is the primer of the following gene loca: Amelogenin, D8S1132, D12S391, GATA198B05, D7S3048, D2S1772, D11S22368, D6S1043 and D13S325.The detection method is carried out in accordance with the following steps: extracting DNA, carrying out compound amplification on gene loca, detecting and determining amplification products.Jointly applied to single parent identification and difficult genetic relationship identification cases with the existing commercialized kits, the invention can enable identification result to be more scientific and act as the beneficial supplement of the existing STR kit.

Description

Technical field [0001] The invention relates to human gene detection, in particular to the analysis of STR (short tandem repeats, short tandem repeats), and specifically to a kit and a detection method for human genotyping using fluorescently labeled STR. Background technique [0002] The STR genetic marker system is widely used in forensic medicine, archaeology, genetics and other fields because it is widely distributed in the human genome, has a high degree of genetic polymorphism and simple and quick detection methods. At present, the commonly used STR commercial kits for personal identification and paternity testing mainly include AmpFLSTRIdentifiler, PowerPlex 16 two kinds. The AmpFLSTR Identifiler system includes 15 autosomal loci: D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, FGA, and a sex identification site Amelogenin; The 16 system includes 15 autosomal loci: Penta E, D18S51, D21S11, TH01, D3S1358, FGA,...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 丛斌李淑瑾谢杨
Owner HEBEI MEDICAL UNIVERSITY
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