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Method for improving survival rate of oysters by high static water pressure treatment

A technology with high hydrostatic pressure and survival rate, applied in fish farming, application, climate change adaptation, etc., to achieve good application prospects, increase survival rate, and improve the effect of breeding success rate

Inactive Publication Date: 2012-05-09
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the application of high hydrostatic pressure to improve the survival rate of biological planting or breeding is only found in the cryopreservation of plant seeds, bacteria, and pig and cow sperm. There is no research on the application of this technology to improve the survival rate of aquatic animal breeding.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Now take one group of parameters wherein as example, set forth the operation method of the present invention as follows:

[0026] 1. Select oyster broodstock with full gonads, and use a microscope to distinguish male and female broodstock;

[0027] 2. Obtain sperm and eggs by artificial insemination, and mix sperm and eggs in a ratio of 10:1 to obtain fertilized eggs;

[0028] 3. The fertilized eggs are cultured in sand-filtered seawater, and each inflatable head fills the sand-filtered seawater with air at an air output of <1L / min;

[0029] 4. Place the fertilized eggs under normal hatching conditions for hatching, and start pressure induction when 50% of the embryos develop to the vegetal larval stage (also known as the D-shaped larval stage);

[0030] 5. Due to the limited space of the hydrostatic pressure instrument (see Chinese patent ZL02241798.2.), in order to obtain a better treatment effect, the oyster embryo is moved into the pressure vessel chamber; first us...

Embodiment 2

[0035] The oyster larvae are at the blastocyst stage, treated with a pressure of 10 MPa for 10 minutes, and the rest of the steps are similar to those in Example 1, and the survival rate after 15 days is 14.7%.

Embodiment 3

[0037] The oyster larvae are gastrula embryos, treated with a pressure of 40 MPa for 10 minutes, and the rest of the steps are similar to those in Example 1, and the survival rate after 15 days is 16.7%.

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PUM

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Abstract

The invention discloses a method for improving the survival rate of oysters by high static water pressure treatment, relating to a culturing method of aquatic animals. Oyster embryos are treated by using a static water pressure; the pressure of the static water pressure is 10-40 MPa; the time for treating the oyster embryos by using the static water pressure is 10-50 min; and the oyster embryos can comprise at least 50% of oyster spermatovums growing to a blastula stage. Before treating the oyster embryos by using the static water pressure, the oyster embryos is filtered and concentrated by using a screen mesh; the concentration of the concentrated oyster embryos can be less than 100 oyster embryos per mL. The aperture of the screen mesh for concentrating the oyster embryos can be 25-60 mu m. The aspects of object, the principle, the method and the like are all obviously different from the conventional methods. The survival rate of the treated oyster embryos is obviously increased, the success rate of culturing the oysters can be greatly improved, and the method has favorable application foreground.

Description

technical field [0001] The invention relates to a method for raising seedlings of aquatic animals, in particular to a method for improving the survival rate of oyster larvae by using high hydrostatic pressure treatment. Background technique [0002] Hydrostatic pressure (hydrostatic pressure) is a common physical parameter in nature. Organisms living in aquatic environments are more or less affected by hydrostatic pressure, but only deep-sea organisms are under high hydrostatic pressure (high hydrostatic pressure, HHP) all the time. ) among. High hydrostatic pressure refers to a hydrostatic environment with a pressure above 10MPa, which is equivalent to the pressure generated by a water depth of 1000m. More than 50% of the earth's surface is covered by oceans with a water depth of more than 1000m, where a large number of biological groups that tolerate high hydrostatic pressure live. Hydrostatic pressure is an important environmental factor affecting the vertical distribut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王德祥梁翠黄建荣虞晋晋柯才焕
Owner XIAMEN UNIV