Gene families of cabbage type rape, parental species Chinese cabbage and cabbage AHA10 thereof and applications thereof

A technology of Brassica napus and gene family, which is applied in the field of genetic engineering and achieves the effect of good application prospect

Inactive Publication Date: 2011-01-12
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, except for the AtAHA10 gene, no other plant AHA10 gene has been cloned, and there are few reports on AHA10 gene research. However, the number of members, protein characteristics, protein characteristics, and Evolutionary relationship, tissue specificity of expression and relationship with yellow seed traits, transgenic molecular breeding, etc. have not been reported

Method used

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  • Gene families of cabbage type rape, parental species Chinese cabbage and cabbage AHA10 thereof and applications thereof
  • Gene families of cabbage type rape, parental species Chinese cabbage and cabbage AHA10 thereof and applications thereof
  • Gene families of cabbage type rape, parental species Chinese cabbage and cabbage AHA10 thereof and applications thereof

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Embodiment Construction

[0039] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the preferred embodiment, generally according to conventional conditions, such as Molecular Cloning Experiment Guideline (third edition, J. Sambrook etc., translated by Huang Peitang, etc., Science Press, 2002) described conditions, or as recommended by the manufacturer.

[0040] The plant materials used in the preferred embodiment: the cabbage materials are all from the cabbage-type rape subspecies (B.rapa ssp.oleifera), including the black seed line 09L597 and the yellow seed line 09L600; the cabbage materials are all from the kale variety (B.oleracea var.acephala), including black seed line 09L598 and yellow seed line 09L599; Brassica napus materials include black seed line 5B and seed color near-isogenic lines (black seed line 09L588, yellow seed line 09L587), all pro...

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Abstract

The invention discloses gene families of cabbage type rape, parental species Chinese cabbage and cabbage AHA10 and applications thereof, wherein the Chinese cabbage AHA10 gene family comprises a BrAHA10-1 gene (SEQ ID No.1-2) and a BrAHA10-2 gene (SEQ ID No.3-4); the cabbage AHA10 gene family comprises a BoAHA10-1 gene (SEQ ID No.5-6) and a BoAHA10-2 gene (SEQ ID No.7-8); the cabbage type rape AHA10 gene family comprises a BnAHA10-1 gene (SEQ ID No.9-10) and a BnAHA10-2 gene (SEQ ID No.11-12); and the gene families can be applied to the molecular breeding of the seed properties of brassica plants.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to Brassica napus and its parent species Chinese cabbage (Brassica rapa) and cabbage (Brassica oleracea) AHA10 (autoinhibited H + -ATPase 10, autoinhibited H + - ATPase 10) gene family and applications thereof. Background technique [0002] The genus Brassica (Brassica) of the Brassicaceae (Brassicaceae) includes many oil crops, vegetables and ornamental plant species. It provides humans with nutritious edible oils, vegetables and ornamental plants, and provides feed for animal husbandry. It has an important economic value. Among the Brassica species, the allotetraploid species Brassica napus was formed from two diploid species Brassica oleracea and Brassica oleracea through interspecific hybridization followed by remultiplication. Brassica napus is the second largest oil crop in the world, widely planted all over the world, and its cultivation area and output are sec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C12N5/10A01H5/00
Inventor 柴友荣冯瑜赵文军陈艳王瑞唐章林李加纳谌利殷家明
Owner SOUTHWEST UNIVERSITY
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