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Method for producing citric acid by fermentation of mixed raw materials

A technology of mixing raw materials and citric acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of storage, unstable composition, difficult sterilization, etc., achieve a large choice, avoid cost rises, The effect that contributes to the improvement of acid production ability

Active Publication Date: 2011-01-19
JIANGSU GUOXIN UNION ENERGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is always pressure to find cheaper, better quality raw materials, and finding new raw materials is always problematic, such as storage, difficult to sterilize, unstable composition

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh the corn raw material, crush it to make corn flour, the fineness is 30 mesh, add water to make corn steep liquor, so that the concentration of corn in the corn steep liquor is 20g / L; input the corn steep liquor into the jet liquefaction device, and adjust the pH to 6.0 , according to 5 enzyme activity units per gram of corn flour, add high-temperature α-amylase to the corn flour slurry, and carry out liquefaction, the temperature is controlled at 100 ° C, and maintained for 5 hours, so that the liquefied DE value is 70%; the liquefied The corn steep liquor is filtered, and the filtrate is retained; the cassava raw material is weighed, cut into pieces and crushed into cassava flour, the fineness is 50 mesh, and water is added to make cassava pulp, and the concentration of cassava in the cassava pulp is 25g / L; the cassava pulp is input into Jet liquefaction device, adjust the pH to 6.5, add high-temperature α-amylase to the cassava flour slurry at 15 enzyme activity ...

Embodiment 2

[0028] Weigh the corn raw material, crush it to make corn flour, the fineness is 40 mesh, add water to make corn steep liquor, so that the concentration of corn in the corn steep liquor is 25g / L; input the corn steep liquor into the jet liquefaction device, adjust the pH to 6.5 , according to 15 enzyme activity units per gram of corn flour, add high-temperature α-amylase to the corn flour slurry, and carry out liquefaction, the temperature is controlled at 105 ° C, and maintained for 3 hours, so that the liquefied DE value is 60%; the liquefied The corn steep liquor is filtered, and the filtrate is retained; the cassava raw material is weighed, cut into pieces and crushed into cassava, the powder fineness is 50 mesh, and water is added to make cassava pulp, and the concentration of cassava in the cassava pulp is 20g / L; the cassava pulp is input into Spray the liquefaction device, adjust the pH to 6.5, add high-temperature α-amylase to the cassava flour slurry at 5 enzyme activi...

Embodiment 3

[0030]Weigh the corn raw material, crush it to make corn flour, the fineness is 40 mesh, add water to make corn steep liquor, so that the concentration of corn in the corn steep liquor is 20g / L; input the corn steep liquor into the jet liquefaction device, and adjust the pH to 6.0 , according to 10 enzyme activity units per gram of corn flour, add high-temperature α-amylase to the corn flour slurry, and carry out liquefaction, the temperature is controlled at 100 ° C, and maintained for 4 hours, so that the liquefied DE value is 80%; the liquefied The corn steep liquor is filtered, and the filtrate is retained; the cassava raw material is weighed, cut into pieces and crushed into cassava flour, the fineness is 60 mesh, and water is added to make cassava pulp, and the concentration of cassava in the cassava pulp is 20g / L; the cassava pulp is input into Jet the liquefaction device, adjust the pH to 6.0, add high-temperature α-amylase to the cassava flour slurry at 10 enzyme activ...

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PUM

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Abstract

The invention discloses a method for producing citric acid by fermentation of mixed raw materials. The method comprises the following main steps of: performing crushing, size mixing, spraying liquefaction and filtration on corn and cassava respectively, then mixing two parts of filtrate according to a proportion and fermenting the mixture, and finally extracting the citric acid. The method effectively avoids cost rise caused by the fluctuation of single raw material, meanwhile enriches the nutrient components of fermented liquor, is favorable for improving the acid producing capability of citric acid fermentation strains, enables an enterprise to have great choice on the raw materials, and reduces the production cost. Moreover, compared with a method for producing the citric acid by the single raw material, the method has the advantages that: the fermentation liquor has good liquidity, the fermentation period is obviously shortened, and the fermentation index is higher.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for fermenting and producing citric acid by using mixed raw materials of corn and cassava. Background technique [0002] The reason why the fermentation industry is superior to other manufacturing industries is that its raw materials can be changed within a certain range, which provides a certain degree of buffer against the increase in raw material prices. For a method that can use different raw materials, its economics play a crucial role in whether the method can be used. The profit margin for citric acid products is passed on to consumers through the increased cost of raw materials. Due to the characteristics of Aspergillus niger, the choice of raw materials for citric acid is less stressful than other fermented products such as antibiotics, so citric acid manufacturers are always looking for raw materials with the lowest possible price. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/48C12R1/685
Inventor 蒋小东胡志杰高金宝孙福新刘振强
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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