Kit and method for detecting riemerella anatipestifer

A technology of Riemer's duck disease and detection kit, which is applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of immune failure, inability to protect ducks against resistance, difficulty in immune prevention, etc., and achieve high specificity , high sensitivity, the effect of shortened reaction time

Active Publication Date: 2011-01-19
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
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Problems solved by technology

Duck infectious serositis has existed for a long time, and it is very difficult to prevent immunization. There are two main reaso...

Method used

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  • Kit and method for detecting riemerella anatipestifer
  • Kit and method for detecting riemerella anatipestifer
  • Kit and method for detecting riemerella anatipestifer

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Embodiment Construction

[0023] The present invention is further described below in conjunction with embodiment, but the present invention is not limited to the content of embodiment.

[0024] (1) Preparation of test samples

[0025] 8 reference strains of RA were obtained from Professor Jiang Shijin of Shandong Agricultural University, including 2 strains of serotype 1, 1 strain of serotype 2, and 5 strains of serotype 10. 306 duck heads of dead ducks were collected from duck farms in Jining, Zaozhuang, Weifang, Linyi, Shandong and Xinyang, Henan, and RA was isolated and purified by bacterial isolation method. The specific method is: aseptically take a little duck brain tissue, spread it on a chocolate plate, and place it in 5% CO 2 Cultivate in the incubator for 24-48 hours, pick out the monoclonal colony similar to the RA colony shape, put it in the nutrient broth medium containing 5% calf serum, cultivate and multiply bacteria overnight in a shaker at 37°C, and obtain Each strain was identified ...

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Abstract

The invention discloses a kit for detecting riemerella anatipestifer and a method for using the kit. At least two pairs of specific primers F3, B3, FIP and BIP for loop-mediated isothermal amplification are placed in the kit for detecting riemerella anatipestifer. For making use convenient, the kit also comprises lycine, magnesium sulfate, dNTPs, ThermoPol reaction buffer and Bst DNA polymerase; or a color developing reagent SYBR GREEN I is also added. The kit and the method have the advantages of convenient application on base layer, short detection time, low cost and simple and convenient operation.

Description

technical field [0001] The invention relates to a kit for detecting bacteria, and methods for its preparation and use, in particular to a kit for detecting Riemerella anatipestifer, and a method for using the kit. Background technique [0002] Riemerella anatipestifer (RA) can cause disease in domestic ducks, geese, turkeys and many other birds, and the resulting duck disease is called duck infectious serositis. The disease was an acute or chronic septic process, and autopsy lesions were characterized by fibrinous pericarditis, perihepatitis, air sac inflammation and caseous salpingitis. In 1932, the disease occurred for the first time in three duck farms in Long Island, New York, USA. The disease was first diagnosed in my country in 1982, and then occurred and became popular in many parts of the country. At present, the disease continues to occur and exist in countries all over the world where the duck industry is developed, causing great harm and economic losses to the du...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 郑福英邱昌庆蔺国珍周继章曹小安宫晓炜
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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