Method for detecting molecular weights and structures of polysaccharide substances by Raman spectrometry
A technology of Raman spectroscopy and molecular weight, applied in the field of polysaccharide compound detection, can solve the problems of long time and high detection cost, and achieve the effect of short time, low detection cost and small sample amount
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Embodiment 1
[0020] 1. Take 20mg of polysaccharide BT-01 in a colorless glass vessel, fix the glass vessel, cover it with a protective cover and avoid light for later use;
[0021] 2. Set up the instrument parameters, use Raman spectrum to test, and get the Raman spectrum of BT-01;
[0022] 3. Perform data processing on the Raman spectrum of BT-01 to obtain the processed Raman spectrum fingerprint of BT-01;
[0023] 4. Comparing the Raman spectral fingerprints of BT-01 and other polysaccharides of the same series, it is obtained that BT-01 distinguishes the molecular weight and structure of other polysaccharides.
Embodiment 2
[0025] 1. Take 20mg of polysaccharide BT-02 in a colorless glass vessel, fix the glass vessel, cover it with a protective cover and avoid light for later use;
[0026] 2. Set up the instrument parameters, use Raman spectrum to test, and get the Raman spectrum of BT-02;
[0027] 3. Perform data processing on the Raman spectrum of BT-02, and obtain the Raman spectrum fingerprint of BT-02 after processing;
[0028] 4. Comparing the Raman spectral fingerprints of BT-02 and other polysaccharides of the same series, the molecular weight and structure of BT-02 can be obtained to distinguish other polysaccharides.
[0029] What needs to be explained here is:
[0030] In the above two embodiments, the instrument parameters of step 2 are as follows: (1) scanning parameters: 10 × 2 × 1 (integration time × scanning times × smoothing coefficient); (2) laser power: 200mW; (3) excitation wavelength : 785nm; the data processing method in step 3 is: adopt multi-wavelength linear regression m...
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