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Application of jewel orchid endophytic fungi to jewel orchid seedlings

A technology of endophytic fungus and clematis, which is applied in the direction of application, fungi, and microorganism-based methods, etc., can solve problems such as unsatisfactory effects, achieve the effects of shortening the growth cycle, enhancing stress resistance, and realizing industrial production

Inactive Publication Date: 2011-01-26
东莞市生物技术研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report abroad on the symbiosis of clematis. Domestically, Guo Shunxing and others used Mycena drobii, Mycena osmundicola, Mycena orchidicola, Mycena anoectochila and Clematis symbiosis (patent application number: 02100779.9; Patent No.: ZL 200510117703.3), effect is not ideal (Gao Weiwei, Guo Shunxing, etc. "Chinese Medicinal Herbs" No. 2002.33 (6) phase "Three kinds of endophytic fungi to Dendrobium officinale, golden thread A Study on the Effects of Lotus Growth)

Method used

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  • Application of jewel orchid endophytic fungi to jewel orchid seedlings
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  • Application of jewel orchid endophytic fungi to jewel orchid seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Firstly, the Rhizoctonia sp. JSH-1 strain was activated from the slant test tube stored at low temperature, then transferred to a PDA plate, and cultured in the dark at 28°C for two days for later use.

[0035] Use a sterilized puncher (0.5-1 cm in diameter) or a burnt inoculation needle to make a block on the edge of the colony or pick a mycelial agar block with a side length of 0.4-0.7 cm.

[0036] Select thicker Fujian clematis tissue culture seedlings with a height of 5±0.5cm, connect them to the symbiotic medium as a whole plant, insert 3 seedlings in each bottle, and pick Rhizoctonia sp. JSH-1 The mycelia agar block was placed between 3 tissue culture seedlings; all the above operations were performed on a sterile ultra-clean bench.

[0037] Culture conditions: temperature: 25°C; light intensity: 1500lx, light time: 12h / d; culture time: 80 days.

[0038] Result analysis:

[0039] 1. The growth status of the tissue-cultured seedlings of Clematis clematis after in...

Embodiment 2

[0047] First of all, make the bacterial agent. The best way to make it is: mix rice bran or bran and water in a weight ratio of 1:1, and adjust the water content to 50% to 60% with PDB (water drops appear but do not flow down) , put it into a sterilized white disk, cover the surface with four layers of gauze, sterilize at 121°C for 1 hour, insert a piece of Rhizoctonia sp. JSH-1 mycelia agar block with a length and width of 0.5cm, and keep it in the dark at 28°C Cultivate for 8 days and grate before use.

[0048] The prepared bacterial agent is evenly mixed with the cultivation substrate according to the weight ratio of 3%, and the cultivation substrate is a mixture of fine peat and perlite (3:1). Then put the cultivation medium mixed with the bacterial agent in a flat sieve with a thickness of 3cm, and plant the clematis tissue culture seedlings that have been hardened for 30 days. The spacing between each plant is 5cm, and the light intensity is controlled between 800~3500lx...

Embodiment 3

[0050] Embodiment three, embodiment four all use Rhizoctonia sp. GXSH-5 to test and analyze respectively according to the steps and conditions described in embodiment one and embodiment two;

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Abstract

The invention relates to application of endophytic fungi, in particular to the application of symbiotic cultivation of jewel orchid endophytic fungi and jewel orchid tissue culturing seedlings. The endophytic fungi are Rhizoctonia spp. and are separated from the root part of the wild jewel orchid by a tissue separation method. The Rhizoctonia spp. is collected in China General Microbiological Culture Collection Center (CGMCC for short), and the address is Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing. The Rhizoctonia spp. infects the jewel orchid tissue culturing seedlings to form symbiotic seedlings, so that the application can improve stress resistance of the jewel orchid tissue culturing seedlings, improve survival rate and shorten growth cycle. Therefore, the production cost is reduced and industrialized production is realized.

Description

Technical field: [0001] The invention relates to the application of an endophytic fungus, in particular to the application of an endophytic fungus of clematis to cultivating seedlings of clematis. Background technique: [0002] Clematis clematis belongs to the perennial herb of the family Orchidaceae. It is a traditional precious medicinal material widely used by the people. It is mostly used to treat tuberculosis, lung heat cough, rheumatoid arthritis, etc. In recent years, the effectiveness of clematis in the treatment of difficult diseases such as hypertension, diabetes and tumors has attracted more and more attention from the medical field. Especially in Taiwan, clematis is known as the "king of traditional Chinese medicine". [0003] However, clematis is very rare in natural resources. The reason is that it has very harsh requirements on the ecological environment and is damaged by small animals and people in nature. The more important thing is that the seeds of clemati...

Claims

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Application Information

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IPC IPC(8): A01G1/00C12N1/14C12R1/645
Inventor 方祥江绮晴郑贵朝
Owner 东莞市生物技术研究所
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