Extracts of panaxtriol saponins and preparation process thereof
A technology of notoginseng triol group and extract is applied in the field of traditional Chinese medicine extract and its preparation, which can solve problems such as poor separation effect, and achieve the effects of reducing liver microcirculation disturbance, less side effects and high curative effect
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Embodiment 1 10
[0057] The preparation of embodiment 1 notoginseng triol group saponins
[0058] Radix Notoginseng, crushed into granules and passed through a 20-mesh sieve, soaked in 8 times of 70% ethanol for 8 hours, installed in a percolation barrel for percolation, collected 30 times of 70% ethanol percolation liquid, and the percolation flow rate was 1BV / h, and the percolation liquid was Concentrate under reduced pressure below 70°C to 1.5 times the medicinal material, centrifuge, put D941 resin on the supernatant, resin and medicinal material 0.75:1g / g, sample loading flow rate is 1BV / h, 7.5 times column volume, flow rate 1.25BV / h, wash with water ; Collect the loading effluent and washing solution, and concentrate the loading effluent and washing solution under reduced pressure to dryness below 70°C for subsequent use. The above dry cream is dissolved in the mobile phase and then put on a C18 reverse phase silica gel column (C18 reverse packing particle size 70 μm ), eluted with 30% a...
Embodiment 3
[0072] Radix Notoginseng, crushed into granules (through a 20-mesh sieve), soaked in 3 times of 70% ethanol for 8 hours, installed in a percolation barrel for percolation, collected 20 times the amount of 70% ethanol percolation liquid, the percolation flow rate is 1BV / h, and percolated The solution was concentrated under reduced pressure below 70°C to 1.5 times of the medicinal material, centrifuged, and the supernatant was loaded with D941 resin (resin and medicinal material 0.5: 1g / g) at a flow rate of 0.5BV / h, 5 times the column volume, and a flow rate of 1BV / h , washed with water; collect the sample effluent and washing solution. The loading effluent and washing liquid were concentrated to dryness below 70°C under reduced pressure, and set aside. The above dry cream was dissolved in the mobile phase and then applied to a C18 reversed-phase silica gel column (C18 reversed packing particle size 30 μm), with 40% acetonitrile and water Elution, eluate below 70 ° C under vacuu...
Embodiment 4
[0074] Radix Notoginseng, crushed into granules (through a 20-mesh sieve), soaked in 10 times of 70% ethanol for 8 hours, installed in a percolation barrel for percolation, collected 40 times the amount of 70% ethanol percolation liquid, the percolation flow rate is 1BV / h, and percolated The liquid was concentrated under reduced pressure below 70°C to 1.5 times of the medicinal material, centrifuged, and the supernatant was loaded with D941 resin (resin and medicinal material 1: 1g / g) at a flow rate of 1.5BV / h, 10 times the column volume, and a flow rate of 1.5BV / h, washing with water; collecting the sample effluent and washing liquid. The loading effluent and washing liquid were concentrated to dryness below 70°C under reduced pressure, and then used for later use. After the above dry cream was dissolved in the mobile phase, it was applied to a C18 reverse phase silica gel column (C18 reverse packing particle size 100 μm), with 50% acetonitrile and water Elution, eluate belo...
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