Method for detecting potential genetic toxicity of organic pollutant in seawater

A technology of organic pollutants and detection methods, which is applied in the field of detection of potential genotoxicity of organic pollutants in seawater, can solve the problems of water environment damage, long retention time of persistent organic pollutants, and difficulty in degradation, and achieves simplified operation steps. Effect

Inactive Publication Date: 2011-02-16
THE FIRST INST OF OCEANOGRAPHY SOA
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Problems solved by technology

The first global study of toxic pollutants in marine mammals has yielded preliminary results, but the results are worrying for sperm whales living in the central Pacific Ocean, which was thought to be unpolluted, reports Science Today. A large amount of persistent organic pollutants (POPs) has accumulated in the body. The results of this

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  • Method for detecting potential genetic toxicity of organic pollutant in seawater
  • Method for detecting potential genetic toxicity of organic pollutant in seawater
  • Method for detecting potential genetic toxicity of organic pollutant in seawater

Examples

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Embodiment 1

[0025] Example 1 The specific implementation of the present invention takes the detection of the potential genotoxicity of the organic pollutant pyrene (Pyrene) dissolved in seawater at a known concentration as an example.

[0026] Take 1mL of pyrene (Pyrene) stock solution with a concentration of 100ug / L, and use 4999mL of sterile ocean water to prepare a positive seawater sample of 0.02ug / L.

[0027] 1) Extraction and concentration of organic pollutants in seawater: extract positive seawater samples with a concentration of 0.02ug / L by liquid-liquid extraction (Liquid-Liquid extraction, LLE) and solid phase extraction (Solid phase extraction, SPE) organic pollutants in seawater, and anhydrous Na 2 SO 4 Dry the column, rinse with dichloromethane to obtain an eluent, blow the eluent to dryness with nitrogen, and finally dissolve it with 1ml of dimethylsulfoxide (DMSO) to obtain an organic concentrate with a concentration of 100ug / L;

[0028] 2) Cell collection and enrichment ...

Embodiment 2

[0038] The detection of embodiment 2 seawater sample

[0039] Examination of the potential genotoxicity of organic pollutants in seawater taken from the Jiaozhou Bay aquaculture area.

[0040] 1) Extraction of organic pollutants in seawater

[0041] 1.1 Collection and processing of seawater samples: According to the requirements for collection of seawater samples in the "Marine Monitoring Specifications", in April 2009, 5L seawater samples were collected from Shazikou shellfish breeding area in Shilaoren, Qingdao, and filled in glass containers. After the sample is collected, it should be sealed immediately, stored at low temperature (below 4°C) and protected from light, and transported back to the laboratory for processing and analysis as soon as possible. If the analysis is not performed immediately, the seawater samples must be stored at low temperature (below 4°C) and in a dark place, and all sample extractions must be completed within 7 days, and the analysis must be com...

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Abstract

The invention relates to a method for detecting the potential genetic toxicity of an organic pollutant in seawater, in particular to a method for detecting the organic pollutant in the seawater by utilizing a marine phytoplankton karenia mikimotoi and single cell gel electrophoresis technology. Marine phytoplankton karenia mikimotoi is used as a biomaterial for detecting the genetic toxicity of the organic pollutant in the seawater, and the karenia mikimotoi has no cell wall, is provided with a large and obvious cell nucleus, is quite sensitive for the change of physical and chemical factors in a water body and is a good biomaterial for researching and evaluating the potential genetic toxicity of the organic pollutant in the seawater on a population level. The method not only can be used for extracting the organic pollutant in the seawater to detect the genetic toxicity thereof, but also can be directly used for detecting the overall genetic toxicity of the seawater in seriously-polluted sea areas, such as a pollutant discharging opening, a culture area and the like. When the overall genetic toxicity of the seawater is detected, the seawater only needs to be filtered to remove silt and impurities for subsequent detection.

Description

technical field [0001] The invention belongs to the technical field of marine pollutant detection, and in particular relates to a detection method for the potential genotoxicity of organic pollutants in seawater. Background technique [0002] With the rapid development of my country's economy and society, a large number and variety of organic pollutants have entered the water environment, causing the water body to show mutagenicity, carcinogenicity and teratogenicity to varying degrees. Since the ocean is the concentrated place of the earth's waters, various organic pollutants will eventually enter the ocean, endangering the marine ecosystem and human health and life safety. The first global study of toxic pollutants in marine mammals has yielded preliminary results, but the results are worrying for sperm whales living in the central Pacific Ocean, which was thought to be unpolluted, reports Science Today. A large amount of persistent organic pollutants (POPs) has accumulat...

Claims

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Application Information

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IPC IPC(8): G01N27/447
Inventor 高春蕾王宗灵王菊英庞敏闫启仑穆景利
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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