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Component recognition method of multicomponent overlapped three-dimensional fluorescence spectrum

A three-dimensional fluorescence, multi-component technology, applied in the fields of environmental science and spectroscopy, can solve problems such as component identification errors

Inactive Publication Date: 2013-09-11
ANHUI INST OF OPTICS & FINE MECHANICS - CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for component identification of multi-component overlapping three-dimensional fluorescence spectra to solve the problem caused by simply considering a certain feature of the two-dimensional spectrum in the decomposition result when using a multi-dimensional analysis algorithm to process seriously overlapping three-dimensional fluorescence spectra. misidentified ingredients

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  • Component recognition method of multicomponent overlapped three-dimensional fluorescence spectrum
  • Component recognition method of multicomponent overlapped three-dimensional fluorescence spectrum
  • Component recognition method of multicomponent overlapped three-dimensional fluorescence spectrum

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Embodiment Construction

[0093] According to the component identification method of the three-dimensional fluorescence spectrum proposed in the present invention, the overlapping spectral signals of the simulation are analyzed as an example,

[0094] In the experiment, three representative emission spectra and excitation spectra were constructed as research objects, with a high degree of overlap. Use these three kinds of emission and excitation spectra to construct the characteristic spectrum database. The characteristic spectra are as follows: figure 2 shown.

[0095] In order to illustrate that the PARAFAC method is affected by multicollinearity and how to eliminate this effect through the comprehensive similarity index, two sets of experimental schemes were selected.

[0096] The first group: select component 1 (component 1) and component 2 (component 2) with high similarity in emission spectrum, and construct 8 groups of different relative concentration scores, see Table 1:

[0097] Table 1: Co...

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Abstract

The invention discloses a component recognition method of multicomponent overlapped three-dimensional fluorescence spectrum, which solves the component recognition error caused by only considering certain characteristic in a two-dimensional spectrum in a decomposition result when a multidimensional analysis algorithm is utilized to process a seriously overlapped three-dimensional fluorescence spectrum. Under the condition of reducing initial solution as much as possible, the characteristic peak and the wave shape characteristic parameter of two two-dimensional fluorescence spectrums decomposed by a multidimensional analytical algorithm are synthetically utilized, the component of the mixed three-dimensional fluorescence spectrum is recognized by building integrated similarity index so as to solve the component recognition problem of overlapped peaks. The automatic search of the similarity matrix formed by the similarity index realizes automatic recognition, and can better adapt to the requirements on continuous and automatic monitoring technology quickly developed in current.

Description

technical field [0001] The invention relates to the fields of environmental science and spectroscopy, in particular to a method for identifying components of multi-component overlapping three-dimensional fluorescence spectra. Background technique [0002] Fluorescence spectroscopy has been widely used in medicine, biology, environmental science, agriculture, chemical industry and other fields because of its high sensitivity and good selectivity. Three-dimensional fluorescence spectroscopy is a new fluorescence analysis technique developed in the 1980s. Three-dimensional fluorescence spectroscopy, also known as total luminescence spectrum, excitation-emission matrix or contour spectrum, can obtain the fluorescence intensity information when the excitation wavelength and emission wavelength change simultaneously, so it can provide more complete spectral information than conventional fluorescence spectroscopy, as A valuable spectral fingerprint technique has been widely used i...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06K9/00
Inventor 肖雪于绍慧张玉钧刘文清王志刚王欢博殷高方段静波
Owner ANHUI INST OF OPTICS & FINE MECHANICS - CHINESE ACAD OF SCI
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