Method for establishing enterovirus 71-type intraperitoneal inoculation infection BALB/c suckling mouse model

An enterovirus and a technology for establishing a method, applied in the direction of medical raw materials derived from viruses/bacteriophages, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve problems such as the lack of stable and mature animal models, and achieve broad application prospects Effect
CN101978970AInactive Publication Date: 2011-02-23INST OF BASIC MEDICINE OF SAMS +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF BASIC MEDICINE OF SAMS
Publication Date
2011-02-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for establishing an enterovirus 71-type (EV71) intraperitoneal inoculation infection BALB / c suckling mouse model. The EV71 infection mouse model is prepared by intraperitoneally injecting virus liquid into a BALB / c suckling mouse. The virus liquid is prepared by the following steps of: inoculating EV71 strains into MA104 cells, performing culture at the temperature of 37 DEG C by using 5 percent carbon dioxide (CO2), and observing the pathological change conditions of the cells every day, wherein on the next day of the EV71 inoculation, agglutination and shrinkage occur on 70 percent of the MA104 cells; and after freeze-thawing the whole bottle of cells, centrifugally taking supernatant to obtain the EV71 virus liquid. By the method, a stable enterovirus EV71 intraperitoneal inoculation infection BALB / c suckling mouse model is established successfully; and foundation is laid for scientific researchers to research EV71 etiology, an EV71 pathogenic mechanism, the screening of high-efficiency anti-virus medicines and the preparation of vaccines. The method has a wide application prospect.
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Description

technical field

[0001] The invention relates to a method for establishing a BALB / c suckling mouse model of enterovirus 71 intraperitoneal inoculation infection. Background technique

[0002] Enterovirus 71 (EV71) belongs to the Enterovirus genus of the picornaviridae family and is one of the main pathogens causing hand, foot and mouth disease (HFMD) in infants. The main symptoms of EV71 infection are rashes or herpes on the hands, feet, mouth and other parts. Some children may develop neurological complications such as aseptic meningitis, brainstem encephalitis, neurogenic pulmonary edema, and polio-like paralysis. Respiratory tract infection and myocarditis etc., can be disabled, lethal. Since the outbreak of hand, foot and mouth disease in Fuyang, Anhui in 2008, the infection of EV71 has been intensified and has become a public health concern. Therapeutic drugs and vaccines.

[0003] The lack of mature and stable animal models is one of the most important reasons for th...

Claims

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