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Duck-origin paramyxovirus attenuated lapinized Chineses (LC) strain and application thereof to the preparation of vaccines

A paramyxovirus, duck-derived technology is applied to the duck-derived paramyxovirus attenuated LC strain and its application in the preparation of vaccines, and can solve problems such as economic losses in the duck farming industry.

Active Publication Date: 2012-06-06
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since 2000, there have been reports of the incidence of muscovy ducks, meat ducks and local breeds of ducks. At present, the disease mainly occurs sporadically in duck raising areas in my country, with an incidence rate of 20-40% and a case fatality rate of about 25%-30%. Cause certain economic losses to the duck industry

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  • Duck-origin paramyxovirus attenuated lapinized Chineses (LC) strain and application thereof to the preparation of vaccines
  • Duck-origin paramyxovirus attenuated lapinized Chineses (LC) strain and application thereof to the preparation of vaccines
  • Duck-origin paramyxovirus attenuated lapinized Chineses (LC) strain and application thereof to the preparation of vaccines

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Embodiment Construction

[0007] Below in conjunction with embodiment and experimental data further illustrate the present invention. (but not a limitation of the invention).

[0008] The attenuated LC strain of duck-derived paramyxovirus (PMV-LC strain) derived from artificial attenuation is artificially selected by biotechnology using the virulent strain of duck-derived paramyxovirus through alternate passages of muscovy duck embryo fibroblasts and chicken embryo fibroblasts. It is formed by breeding and weakening, and has independent intellectual property rights. The PMV-LC strain has lost its pathogenicity to one-day-old young muscovy ducks, and has the following characteristics (1) virus titer: the PMV-LC strain is inoculated through the allantoic cavity on 9-day-old chick embryo ELD 50 ≥10 -4.5 / 0.1ml, allantoic fluid HA titer ≥ 2 -8.0 ; To chicken embryo fibroblast monolayer cells TCID 50 ≥10 -5.5 / 0.1ml; (2) safety: use 10 feathers of 1-day-old healthy susceptible young muscovy ducks, inje...

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Abstract

The invention relates to an aritificial-attenuated duck-origin paramyxovirus attenuated lapinized Chineses (LC) strain. The strain is collected in China Center for Type Culture Collection (CCTCC), the collection date is June 2nd, 2010, and the collection number is CCTCC NO: V201018. The invention also comprises the application of the duck-origin paramyxovirus attenuated LC strain to the preparation of duck-origin paramyxovirus disease live vaccines, adjuvant inactivated vaccines and subunit vaccines. The aritificial-attenuated duck-origin paramyxovirus attenuated LC strain is suitable for the large-scale production of novel duck-origin paramyxovirus disease vaccines under the condition of good manufacturing practice (GMP).

Description

technical field [0001] The present invention relates to attenuated LC strain of duck-derived paramyxovirus and its application in vaccine preparation. Background technique [0002] Paramyxovirus (APMV) belongs to the Paramyxoviridae, Paramyxoviridae, and Mumps virus genus. There are currently nine serotypes identified, namely from APMV-1 to APMV-9, of which APMV-1 is the most important in poultry. Newcastle disease virus is the representative species of APMV-1. Previous studies have reported that waterfowl (ducks, geese) have extremely strong resistance to pathogenic avian paramyxovirus type 1 (APMV-1), and they only appear to be infected. However, since 2000, there have been reports of the incidence of muscovy ducks, meat ducks and local breeds of ducks. At present, the disease mainly occurs sporadically in duck raising areas in my country, with an incidence rate of 20-40% and a case fatality rate of about 25%-30%. Cause certain economic loss to duck industry. In order to...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/155A61P31/14C12R1/93
Inventor 陈少莺程晓霞俞伏松陈仕龙朱小丽林锋强黄梅清王劭
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI