Detection test paper for Francisella tularensis antibody, preparation method thereof, detection method utilizing detection test paper and quantitative detection system

A technology for detecting test paper and soil pull, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of limited popularization and application, environmental pollution, operator safety, etc., and achieve the effect of simple operation and short detection time

Inactive Publication Date: 2011-03-02
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
  • Description
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Problems solved by technology

However, lipopolysaccharide has defects in large amount of acquisition, environmental pollution and operator safety, which limits the promotion and application of these methods.

Method used

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  • Detection test paper for Francisella tularensis antibody, preparation method thereof, detection method utilizing detection test paper and quantitative detection system
  • Detection test paper for Francisella tularensis antibody, preparation method thereof, detection method utilizing detection test paper and quantitative detection system
  • Detection test paper for Francisella tularensis antibody, preparation method thereof, detection method utilizing detection test paper and quantitative detection system

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[0031] Such as Figure 1 to Figure 4 , Figure 5A , Figure 5B , Figure 6A , Figure 6B , Figure 6C as shown, figure 1 It is the visual sensitivity diagram of the detection test strip of the present invention. 1: Negative control; 2-7: Tularemia antibody concentration 2400ng / mL, 1200ng / mL, 120ng / mL, 60ng / mL, 30ng / mL, 15ng / mL.

[0032] figure 2 For specific detection test. Test strips 1-6: 1ug / mL anti-Ma Qiubo GP2 antibody, smallpox M1R antibody, yellow fever YF-E-D3 antibody, avian influenza NP antibody, dengue virus Deng-E-D3 antibody and Rickettsia 120N Antibody

[0033] image 3 It is the stability detection of the visual sensitivity diagram of the detection test strip of the present invention; test strip 1: negative control, 2-4: tularemia antibody concentration 120ng / mL, 60ng / mL, 30ng / mL

[0034] Figure 5A , Figure 5B Middle, 1: absorbent pad; 2: nitrocellulose membrane, T: detection band coated with recombinant FopA protein; C: quality control band coa...

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Abstract

The invention discloses a detection test paper for Francisella tularensis antibody, a preparation method thereof, a detection method utilizing the detection test paper and a quantitative detection system, the rapid detection method for the Francisella tularensis antibody is established by utilizing the colloid gold labeling technique and double antibody sandwich immunochromatographic technology, and the established colloid gold immunochromatographic technology for detecting the Francisella tularensis antibody can rapidly, sensitively, specifically and accurately detect the Francisella tularensis antibody in a sample, realize quantitative detection and be applicable to field rapid detection.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a test paper for Francisella tularensis antibody detection and a preparation method thereof, a detection method and a quantitative detection system using the test paper. Background technique [0002] Tularaemia is an acute infectious zoonotic disease. Its causative bacterium is Francisella tularensis. The susceptible animals are rodents. Humans are infected through damaged skin and conjunctiva. , Inhalation or ingestion of a very small amount (10-50) of tularemia can cause the disease. The clinical manifestations are skin ulceration, enlarged lymph glands, lung infection and typhoid-like symptoms, and the condition is severe and even death. [0003] At present, when the double-antigen sandwich colloidal gold method used in China detects tularemia, lipopolysaccharide (LPS) of tularemia is commonly used as the antigen for detecting antibodies in serum. However, lipo...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543
Inventor 王静景滢滢杨宇杨永莉孙肖红胡孔新
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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