Method and device for detecting dentin phosphoprotein

Abstract

The invention discloses a method and a device for detecting dentin phosphoprotein, which belong to the technical field of electrochemical enzyme-linked immunosorbent assays. In a device in which an electrolytic bath, a detecting instrument and a mercury storage bottle are connected in series and an external power supply is connected with the detecting instrument, the accurate content of the dentin phosphoprotein is accurately measured by combining a 'double antibody sandwich method' enzyme-linked immunosorbent assay and an 'electrochemical' detection method, the dentin phosphoprotein in buccal gingival crevicular fluid of patients is extracted by adopting a 'filter paper absorption method', the dentin phosphoprotein in the buccal gingival crevicular fluid of patients is purified by adopting a 'protein extract purification method', and the purified dentin phosphoprotein is put into a centrifugally sealed tube and is taken as a sample to be detected. In the invention, the accurate detection of a change rule of the dentin phosphoprotein in the gingival crevicular fluid can be realized and the early diagnosis of dental root resorption of patients can be realized; and the detection method has the advantages of simpleness in operation, high sensitivity, no radiation damage and the like, and has good application prospect in the clinical diagnosis of the dental root resorption.

Description

technical field [0001] The invention belongs to the technical field of electrochemical enzyme-linked immunoassay. In particular, it relates to a dentin phosphoprotein detection method and a device thereof for early diagnosis of tooth root resorption based on electrochemical enzyme-linked immunoassay technology. Background technique [0002] Most patients experience mild root resorption during the orthodontic process, and there will be no tooth loosening after the end of the orthodontic treatment. However, a small number of patients will experience severe root resorption, and the teeth will become loose or even fall out; orthodontic root resorption is the main complication of orthodontic treatment. If root resorption can be diagnosed early, it can prevent the patient's tooth from becoming loose or falling out. At present, imaging methods such as curved surface tomography, root tip radiograph, and CT are mainly used to diagnose tooth root resorption. It is urgent to find a m...

AI Technical Summary

Problems solved by technology

However, due to the small amount of gingival crevicular fluid samples and the rare active ingredients in the liquid to be tested, it is difficult to ensure the uniformity and light transmittance of the enzyme-linked plate, and the use of spectrophotometry limits the precision and detection limit of the assay, which is easy False-negative results occur, and there is currently no effective way to solve the above problems

Images