Method for making globefish-inflated sample and application thereof

Abstract

The invention relates to a method for making a globefish-inflated sample and application thereof. The method comprises the following steps of: (1) selecting globefish with the same age in days and similar body length, volume and weight so as to guarantee the same water sucking time and lethal time for achieving the expected effect; (2) measuring the time for the globefish to suck water and inflate to the greatest degree; (3) determining the volume of the globefish before death and after inflation; (4) determining the needed lethal concentration of an anesthetic; (5) making the globefish inflated to die; and optionally (6), checking whether the globefish is inflated to the greatest degree. The inflation degree of the globefish can be controlled by an anesthetic lethal method, and compared with the conventional method, the method has the greatest characteristic that: the probability for obtaining the fish inflated to the greatest degree is obviously improved, and whether the finish is inflated to the greatest degree can be judged. In addition, the operation is easy to operate, special and expensive medicines or instruments are not needed, the anesthetic lethal method meets the requirement on the death of finish internationally, the operation can be completely finished in a laboratory, the time and cost are saved, and the method contributes to quickly finishing an experiment.

Description

technical field [0001] The invention relates to a method for making a fish specimen and its application. Specifically, the present invention relates to a preparation method and application of puffer fish flatulence specimen. Background technique [0002] Due to the difference in living habits and living environments, fish of different sizes have very different movement patterns. Fish with a spindle shape swim faster. The head muscles and the unilateral body and tail muscles produce strong contractions, causing the fish to escape danger in a "C" or "S" shape. However, the fishes of the family Pufferidae and Pufferidae in the order Pufferiformes are thicker, cylindrical, and slow in swimming speed and slow in movement, but they can make up for their slow swimming speed in other special ways. When encountering stimuli ( or predators), it can quickly inhale air and water, making the chest and abdomen swell like a ball, and the small spines on the skin stand up so that the fish...

AI Technical Summary

Problems solved by technology

However, there are two problems with the fish body obtained in this way: one is that it died due to unknown reasons; the other is that the probability of being at the limit of flatulence is very small, and because the location is far away from the researcher, it is inconvenient to obtain materials and is not conducive to the research;

However, the experiment found that the fish reacted violently when they were put into the formalin solution with four to five times the normal concentration, but the probability of flatulence was very small. When more than 50 puffer fish were fixed in the formalin solution at different concentrations, only Obtained 5 fish in the state of flatulence, but not fish at the limit of flatulence, and the direct fixation with formalin is too painful for the fish, while in foreign countries, deep anesthesia is often used to kill the fish to reduce the pain of the fish

[0007] Third, by injecting liquid nitrogen into the instant death, that is, after the six-class spinyfish reaches the limit of flatulence, it is quickly put into liquid nitrogen to keep it in a state of flatulence. This method can be controlled by researchers before, but when it is put into Due to the different sizes of fish after liquid nitrogen, the time for liquid nitrogen to kill them is different, that is, the flatulence state is not easy to control, and because liquid nitrogen is required to kill, the conditions are high and dangerous

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