Celery cytoplasmic male sterile line breeding method and cross-breeding method using celery cytoplasmic male sterile line

Abstract

The invention discloses a celery cytoplasmic male sterile line breeding method and a cross-breeding method using a celery cytoplasmic male sterile line. In the methods, celery '021542' serving as a female parent and chervil '022047' serving as a male parent are subjected to manual control hybridization, male sterile plants are selected from an F1 group as a cytoplasmic male sterile source and is subjected to fifth backcross generation with the celery '021542' to obtain a celery cytoplasmic male sterile line '0863A'; and a backcross male parent corresponding to the celery cytoplasmic male sterile line is a maintainer line '0863B'. The sterile line '0863A' and a selected male parent line are subjected to field planting in a cross-breeding farm with an excellent isolation condition in a ratio of 3:1; and after pollination is finished, male parent plants are uprooted completed, and seeds picked from sterile line plants after the yellow maturity of the seeds are generation hybrids. The cytoplasmic male sterile line bred by the method has stable heredity, high combining ability, simple cross-breeding process, low cost and high quality of the hybrids, and can be used for the large-scale cross-breeding production of celery.

Description

Background technique [0001] Celery is a biennial vegetable of the genus Celery in the family Umbelliferae. It is rich in protein, vitamins, mineral elements and other nutrients. Celery is native to the Mediterranean coast. It was artificially cultivated in my country in the Han Dynasty, and it has become one of the most important vegetable crops in my country. Celery is also commonly cultivated in many European and American countries. However, in the long-term celery production, people can only use conventional varieties, and there are no hybrids. The main reason is that celery seed plants have small flowers, large numbers of flowers, and scattered flowering stages, so strict artificial detasseling cannot be carried out, and it is difficult to achieve artificial hybrid seed production. [0002] The use of male sterile lines for hybrid seed production has now become an important way for the breeding of many new crop varieties. There are still few research reports on male ster...

AI Technical Summary

Problems solved by technology

But along with the continuous deepening of breeding practice, the inventor finds that there are obvious deficiencies in 200510013213.9: the first is that the identification of sterile plants and fertile plants must be carried out according to past experience at the initial stage of flowering during hybrid seed production, which just Increased technical difficulty and limited t...

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