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Ectomycorrhizal fungi efficiently symbiotic with pinus koraiensis

An ectomycorrhizal and Korean pine technology, applied in fungal products, horticulture, botanical equipment and methods, etc., can solve the problems of slow growth, yellow leaves, and low biomass.

Inactive Publication Date: 2011-04-13
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the defects of annual Korean pine seedlings in artificial cultivation of Korean pine seedlings, slow growth, low biomass, low absorption rate of nutrients such as nitrogen, phosphorus and potassium, small stems, withered and yellow leaves, and low survival rate, the present invention provides a plant Ectomycorrhizal Fungus Efficiently Symbiotic with Korean Pine

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  • Ectomycorrhizal fungi efficiently symbiotic with pinus koraiensis
  • Ectomycorrhizal fungi efficiently symbiotic with pinus koraiensis

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specific Embodiment approach 1

[0011] Specific embodiment one: the ectomycorrhizal fungus of this embodiment and Korean pine efficient symbiosis is boletus (Suillus sp.) HDSF2, is preserved in China Microorganism Culture Preservation Management Committee General Microorganism Center, preservation number is CGMCC No.4175 .

[0012] The fruiting body of boletus sp. (Suillus sp.) HDSF2 in the present embodiment is medium to large, and the cap diameter is 6-12 cm, oblate hemispherical, rear central convex, sometimes central concave, smooth, sticky, and the color is reddish brown. The flesh of boletus HDSF2 is light yellow; the initial color of the tube is light, then it becomes light grayish yellow, and the wound becomes lavender or brownish, straight to nearly elongate; the tube mouth is small, angular, partly compound, 1~ per mm 2; stalk length 6-12cm, diameter 1-2.6cm, sub-cylindrical, slightly equal thickness at the top and bottom or slightly thinner at the base, no glandular spots, and reticulate at the to...

specific Embodiment approach 2

[0015] Specific embodiment two: this embodiment will be divided into 2 groups at random with the Korean pine nursery stock that grows 54 weeks on the laboratory sterilization substrate, the first group of Korean pine seedling inoculation specific embodiment one boletus HDSF2, each Korean pine seedling cattle Hepatitis HDSF2 inoculum volume is 120mL. The second group of Korean pine seedlings was used as a blank control group (not inoculated with boletus HDSF2). The rest are the same as the artificial planting and cultivation methods of conventional Korean pine seedlings.

[0016] The present embodiment adopts punching to inject and inoculate boletus HDSF2, around every red pine seedling (hole and seedling distance<5cm) hole injection 4 places, every hole injection 30mL boletus HDSF2 nutrient solution. The concentration of boletus HDSF2 mycelium in the culture solution is: mycelium dry weight 12g / L.

[0017] 20 weeks after boletus HDSF2 inoculation, the infection rate of bolet...

specific Embodiment approach 3

[0024] Specific embodiment three: the present embodiment will " Daqingshan ectomycorrhizal fungus culture characteristic and Pinus tabulaeformis mycorrhiza synthesis research " (author Lu Lijun, " Inner Mongolia Agricultural University " 2005) the boletus sclerosus ( Suillus grevillei), Suillus subluteus and specific embodiment one boletus HDSF2 carry out the Korean pine symbiosis experiment together.

[0025] The inoculation method is the same as in the second embodiment, and the rest are the same as the artificial planting and cultivation methods of conventional Korean pine seedlings.

[0026] After 20 weeks of inoculation, the infection rate of Suillus grevillei was 68.56%. The subbrown-ringed boletus (Suillus subluteus) did not establish a symbiotic relationship with Korean pine, and the infection rate of Suillus sp. HDSF2 The rate is 86.42%.

[0027] After 20 weeks of inoculation and growth of Korean pine seedlings, various indicators were measured, and the test results ...

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Abstract

The invention discloses ectomycorrhizal fungi efficiently symbiotic with pinus koraiensis, relates to the ectomycorrhizal fungi, and overcomes the defects that the conventional artificial breeding annual pinus koraiensis seedlings are short, grow slowly and have low biomass, low absorption rate of nitrogen, phosphorus, potassium and other nutrient substances, small stalks, yellow leaves and low survival rate. The ectomycorrhizal fungi efficiently symbiotic with the pinus koraiensis are suillus sp. HDSF2 and collected in China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.4175. The suillus sp. HDSF2 can be used for the artificial breeding of the pinus koraiensis.

Description

technical field [0001] The invention relates to an ectomycorrhizal fungus. Background technique [0002] Korean pine, also known as fruit pine, belongs to the Pinaceae plant and is a national Class II key protected wild plant. It is mainly distributed in the area from Changbai Mountain to Xiaoxing'anling in northeast my country. Korean pine is a precious and ancient tree species. Pine nuts have high nutritional value and medical and health care value, and have great potential for development; and as a precious economic tree, Korean pine has light and soft materials, is not easy to deform, and has strong corrosion resistance. It is suitable for construction , bridges, sleepers, furniture making, etc. Because of the high economic value of Korean pine, after 56 years of logging since the development of Xiaoxing’an Mountains in 1948, the natural Korean pine forest has been reduced from the original 1.2 million hectares to less than 50,000 hectares, and only about 3 million mat...

Claims

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Application Information

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IPC IPC(8): A01H15/00A01G1/04
Inventor 宋福强孟剑侠
Owner HEILONGJIANG UNIV