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Method for preparing patterned agarose stamp

An agarose, patterned technology, applied in printing, stamping and other directions, can solve the problems of unreported applications, and achieve the effects of fast dissolution, good transparency, and easy availability of raw materials

Active Publication Date: 2012-05-23
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Agarose has been widely used as a gel in electrophoresis, but the application of patterned agarose stamps has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Place the patterned polydimethylsiloxane (PDMS) stamp and the n-hexane solution of 1H, 1H, 2H, 2H-perfluorooctyltrichlorosilane in an airtight container, and vacuum to make 1H, 1H, 2H , 2H-perfluorooctyltrichlorosilane volatilizes, and gas-phase fluorination at room temperature for 2 hours to obtain a fluorinated polydimethylsiloxane seal with a pattern;

[0026] Configure 8% agarose aqueous solution, cast into fluorinated PDMS stamp with pattern at room temperature; quickly heat up to 80 °C for half an hour, take it out, and cool it to room temperature; take out the sol-gel agarose and peel it off from the PDMS stamp , to get a patterned agarose stamp.

[0027] Seals are stored in a constant temperature and humidity box (temperature 15°C, humidity 60%) to prevent drying in the air.

Embodiment 2

[0029] Place the patterned polydimethylsiloxane (PDMS) stamp and the n-hexane solution of 1H, 1H, 2H, 2H-perfluorooctyltrichlorosilane in an airtight container, and vacuum to make 1H, 1H, 2H , 2H-perfluorooctyltrichlorosilane volatilizes, gas phase fluorination at room temperature for 5 hours to obtain a fluorinated polydimethylsiloxane seal with a pattern;

[0030] Configure a 10% agarose aqueous solution, cast it into a fluorinated PDMS stamp with a pattern at room temperature; heat it up to 93 °C for 1 hour, take it out, and cool it to room temperature; take out the sol-gel agarose and peel it off from the PDMS stamp , to get a patterned agarose stamp.

[0031] Seals are stored in a constant temperature and humidity box (temperature 14°C, humidity 55%) to prevent drying in the air.

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PUM

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Abstract

The invention discloses a method for preparing a patterned agarose stamp, wherein the method comprises the steps of fluorinating a polydimethylsiloxane (PDMS) stamp with patterns at room temperature, casting aqueous agarose solution to the fluorinated PDMS stamp with the patterns at room temperature, increasing the temperature to 80 to 90 DEG C, keeping the stamp for a while, taking out the stamp, cooling the stamp to the room temperature and removing solated agarose which is peeled from the PDMS stamp, so as to obtain the agarose stamp with the patterns. The agarose stamp is clear in patternand large in pattern area and can be preserved for a long time.

Description

technical field [0001] The invention relates to a preparation method of agarose patterned seals. Background technique [0002] Agar, also known as agar, frozen powder, is a kind of algin extracted from A. lily and other red algae plants. It has a history of more than 300 years in my country and Japan. Agar is composed of agarose and agaropectin. Agarose is a linear polymer whose basic structure is 1,3-linked β-D-galactofuranose and 1,4-linked 3,6-anhydroα-L-galactofuraanose. Agar pectin is a heterogeneous mixture composed of many smaller molecules, but with sulfate and carboxyl components, and has poor gelling ability. Agarose has been widely used in food, medicine, chemical industry, textile and other fields because of its special gelling properties, especially remarkable stability, hysteresis and hysteresis, and easy to absorb water. [0003] Agarose is generally heated to above 90 °C to dissolve in water, and a good semi-solid gel is formed when the temperature drops t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B41K1/00
Inventor 周峰刘盈王道爱郝京诚
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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