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Aseptic simple liquor relief device of cell culture liquid

A technology of pipetting device and cell culture, applied in biochemical cleaning devices, enzymology/microbiology devices, biochemical instruments, etc., can solve the problems of cumbersome and time-consuming operation methods, unclean liquid absorption, and waste liquid tank pollution, etc. Eliminate man-made pollution, absorb cleanly and thoroughly, and absorb accurately

Inactive Publication Date: 2011-05-18
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional operation method is cumbersome and time-consuming, and due to the limitation of the suction nozzle of the glass straw, the liquid will not be sucked cleanly, and the repeated dumping of the waste liquid tank is also prone to artificial pollution and other disadvantages.

Method used

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  • Aseptic simple liquor relief device of cell culture liquid
  • Aseptic simple liquor relief device of cell culture liquid
  • Aseptic simple liquor relief device of cell culture liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] When carrying out suspension cell subculture in embodiment 1:

[0015] Step 1: Burn the sharp mouth of the gun tip connector, insert a sterile gun tip when the temperature drops, and place it in the air.

[0016] Step 2: Transfer the cells in the cell culture flask to a 15ml centrifuge tube with a pipette, and centrifuge to pellet the cells. Step on the air pump, release it, the tip of the gun is inserted into the liquid in the centrifuge tube, and the liquid is drawn back into the waste liquid collection bottle.

[0017] Step 3: Draw a certain amount of PBS buffer solution into the centrifuge tube, blow and aspirate the cells several times, after centrifugation, replace the tip with the tip connector and draw the liquid to the waste liquid collection bottle according to the above operation.

[0018] Step 4: Draw the fresh culture solution into the centrifuge tube, gently pipette and mix, and then distribute it equally to two culture bottles.

[0019] Table 1 adopts t...

Embodiment 2

[0021] When embodiment 2 carried out subculture of adherent cells:

[0022] Step 1: Burn the sharp mouth of the gun tip connector, insert a sterile gun tip when the temperature drops, and place it in the air.

[0023] Step 2: Step on the air pump, release it, put the tip of the gun into the liquid in the cell culture bottle, and the liquid is drawn back into the waste liquid collection bottle.

[0024] Step 3: Draw a certain amount of PBS buffer solution into the culture bottle, gently blow and wash several times, replace the tip of the tip connector, and then draw the liquid to the waste liquid collection bottle according to the above operation. Repeat this step to ensure minimal influence of serum during trypsinization.

[0025] Step 4: Draw a small amount of trypsin digestion solution into the culture bottle, digest it for 1 minute, then draw fresh culture solution into the culture bottle to stop the action of trypsin to prevent damage to the cells. Gently pipette, move t...

Embodiment 3

[0029] When carrying out cell lysis in embodiment 3,

[0030] Step 1: Burn the sharp mouth of the gun tip connector, insert a sterile gun tip when the temperature drops, and place it in the air.

[0031] Step 2: Step on the air pump, release it, put the tip of the gun into the liquid in the cell culture bottle, and the liquid is drawn back into the waste liquid collection bottle.

[0032] Step 3: Draw a certain amount of PBS buffer solution into the culture bottle, gently blow and wash several times, replace the tip of the tip connector, and then draw the liquid to the waste liquid collection bottle according to the above operation. Repeat this step to ensure minimal influence of serum during trypsinization.

[0033] Step 4: Draw the trypsin digestion solution into the culture bottle, digest for 1 minute and then draw fresh culture solution into the culture bottle to stop the action of trypsin to prevent the cells from being damaged. Gently pipette, move to a 15ml centrifuge...

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Abstract

The invention relates to an aseptic simple liquor relief device of a cell culture liquid, which is characterized in that a rubber hose is connected with a spearhead connector first, then respectively connected with upper pipe orifices of two intubation tubes inserted into a waste liquid collecting bottle from a plug, and then connected with a gas divider and a gas pump. The front end of the spearhead connector is contracted and thinned so as to insert the tenuous spearhead. The gas divider is a three-way pipe in a y-shaped structure. Closable elastic membranes with V-shaped sections and upward closed angles are respectively in the middle lower portion of the long dash and in the middle portion of short dash of the y-shaped structure. The included angle between the long dash and the short dash of the y-shaped structure is ordinarily 30-60 degrees, preferably 45 degrees. The aseptic simple liquor relief device of the cell culture liquid makes the liquid absorption process more accurate and efficient and is simpler and faster for operation; and the liquid is absorbed cleanly and thoroughly, thereby eliminating the corrupt practices of artificial pollution and the like.

Description

technical field [0001] The invention is an aseptic transfer facility for cell culture fluid and other liquids in the ultra-clean bench between cells. Background technique [0002] In the process of cell culture, operations such as washing cells, digesting cells, and replacing culture medium are often performed to ensure the normal growth and subculture of cells, and the requirements for sterility are very strict. The current method is to use a new sterilized glass pipette to suck out the cell washing solution and the old culture solution every time the liquid is changed, and then replace the sterile pipette to suck the new culture liquid into the culture bottle, and the sucked liquid should be put into the culture bottle temporarily. The waste liquid tank in the ultra-clean bench, take it out after the experiment is over, dump it and put it back into the ultra-clean bench. This traditional method of operation is cumbersome and time-consuming, and the limitation of the sucti...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12M33/04
Inventor 王旭李文哲
Owner DALIAN UNIV
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