Device for pretreatment of amino acid test sample

Abstract

The invention discloses a device which is used in a laboratory for the pretreatment of an amino acid test sample. The device is composed of a distributing pipe 1, a first buffer gas-distributing bottle 2, a gas-washing bottle 3, a drying bottle 4, a second buffer bottle 5 and a vacuum source 8, wherein pipelines are respectively used between the distributing pipe 1 and the first buffer gas-distributing bottle 2, between the first buffer gas-distributing bottle 2 and the gas-washing bottle 3, between the gas-washing bottle 3 and the drying bottle 4, between the drying bottle 4 and the second buffer bottle 5, and between the second buffer bottle 5 and the vacuum source 8 for communication; the distributing pipe 1 is also provided with a pipeline 21 which is communicated with a nitrogen source 9; the pipeline 21 and the pipeline communicating the distributing pipe 1 and the first buffer bottle 2 are separately provided with a valve 17 and a valve 13; and the pipeline 21 is also provided with a flow-limiting capillary tube 11.

Description

technical field [0001] The invention relates to equipment used in a laboratory, a device for pretreatment of amino acid detection samples, more precisely, a device for vacuuming, filling with nitrogen and hydrolyzing the amino acid liquid to be tested, followed by constant temperature vacuum drying. Background technique [0002] In the full detection of amino acids, the sample must be hydrolyzed first to break the polypeptide chain of the protein so that the amino acids exist in the form of monomers. This hydrolysis process must be carried out under anaerobic conditions to prevent the loss of sulfur-containing amino acids, tryptophan, tyrosine, histidine, etc. due to oxidation. There is no such device for pretreatment of amino acid detection samples in the existing experimental instruments, and the current laboratory uses self-made equipment to carry out related operations. For this reason, amino acid samples at this stage often suffer from amino acid oxidation loss caused ...

AI Technical Summary

Problems solved by technology

For this reason, amino acid samples at this stage often suffer from amino acid oxidation loss caused by air entry after treatment. In addition, it is not easy to control the temperature when the hydrolyzed solution is dried to prepare the sample solution on the machine. If the temperature is too high, some amino acids will be destroyed.

Therefore, at this stage, after the amino acid sample is processed in the laboratory, some amino acids are often oxidized, which makes the coefficient of variation of the test results between laboratories larger. Experimental data show that the actual measured coefficient of variation (CV%) of cystine and methionine is 41.38%, 22.06%

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