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Method for identifying and screening barley low-nitrogen resistant property

A barley and trait technology, applied in the fields of botanical equipment and methods, horticulture, soilless culture, etc., can solve problems such as no evaluation and screening

Inactive Publication Date: 2011-06-29
SHANGHAI ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of these combined low-nitrogen tolerance-related traits and yield traits in barley seedlings to evaluate and screen for low-nitrogen tolerance.

Method used

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  • Method for identifying and screening barley low-nitrogen resistant property
  • Method for identifying and screening barley low-nitrogen resistant property
  • Method for identifying and screening barley low-nitrogen resistant property

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1: Evaluation and Screening of Barley Seedling Tolerance to Low Nitrogen Using Solution Test

[0079] The solution culture experiment was carried out in the laboratory greenhouse, and the nutrient solution configuration method was referred to the nutrient solution formula of the International Rice Institute (slightly modified). The seeds are sterilized with 1% NaClO for 30 minutes, rinsed with clean water, soaked at 30°C for 5 hours, germinated overnight at 25°C, and sown into vermiculite sterilized by high temperature and high pressure after dew whitening. After removing the endosperm, fix it on a perforated foam board with sponge strips, then put it into a turnover box (40cm×27cm×10cm) filled with clean water, and replace it with nutrient solution after two days of recovery, with an average of 30 on each foam board. Wells (7×5), set 3 varieties, 5 plants for each variety, set 2 treatments of low nitrogen (0.4mmol / L) and normal nitrogen supply (2mmol / L), with N...

Embodiment 2

[0088] Example 2: Evaluation and Identification of Barley's Tolerance to Low Nitrogen at Maturity Stage by Field Test

[0089]The field test was carried out on the farm of the test base. The site of this test is a rice and wheat rotation field. The soil is loamy. The surface 20 cm thick soil contains 34.5g / kg of organic matter, 2.42g / kg of total nitrogen, and 37.35mg / kg of available nitrogen. The experiment adopted a split-plot design, the main plot was fertilizer treatment, and two treatments were set up, namely, normal application of nitrogen fertilizer and no application of nitrogen fertilizer. The sub-plot consisted of 3 varieties, and the area of ​​each plot was about 7.5 square meters, with 3 repetitions. After maturity, the barley kernels were harvested and the yield measured. In this way, the true and reliable evaluation and identification of barley low nitrogen tolerance can be carried out. The barley used is the same variety as the barley used in the seedling experi...

Embodiment 3

[0093] Example 3: Variation Analysis of the Relative Values ​​of Low Nitrogen Tolerance in Barley Seedling and Mature Stages

[0094] The relative value of low-nitrogen tolerance traits at seedling stage was calculated by taking the trait value under low nitrogen stress at the seedling stage / the trait value under normal nitrogen supply, and the grain yield under no fertilization at the mature stage of barley / the grain yield under normal fertilization was The relative value of barley tolerance to low nitrogen, the higher the value, the stronger the tolerance to low nitrogen. See the experimental results figure 1 and 2 .

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Abstract

The invention provides a method for screening and identifying a barley low-nitrogen resistant property. The method comprises the following steps of: screening and identifying a low-nitrogen resistant property in a seedling stage by a solution culture method; screening and identifying the low-nitrogen resistant property in a mature stage by a field experiment; and evaluating the barley low-nitrogen resistant property effectively by establishing the correlativity between the seedling stage and the mature stage, so that the barley low-nitrogen resistant property can be screened and identified quickly and effectively during the seedling stage. The implementation method provided by the invention is simple, a laboratory part is precise and controllable, and a field part is real and accurate; thus, results are effective and reliable.

Description

technical field [0001] The invention belongs to the technical field of barley breeding, and relates to the screening and identification of low-nitrogen tolerance traits of barley with seeds as the propagation mode. Background technique [0002] Barley is the main raw material for making beer and is also an excellent feed. In recent years, with the rapid development of my country's beer industry, the demand for malting barley is increasing. Nitrogen is an essential nutrient element for crop growth. In order to ensure high and stable crop yield, a large amount of nitrogen fertilizer must be applied. In recent years, with the large-scale use of nitrogen fertilizer, while promoting the increase and stability of crop production, it has also brought many disadvantages, such as the decline of economic benefits, the reduction of nitrogen utilization rate, environmental pollution, etc., not only caused huge economic losses, but also Seriously pollute the environment and pose a pote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G1/00
Inventor 陈志伟黄剑华陆瑞菊王亦菲何婷
Owner SHANGHAI ACAD OF AGRI SCI
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