Sampling method for non-damage fixed position observation of plant stomata in field experiment

Abstract

The invention discloses a sampling method for non-damage fixed position observation of plant stomata in a field experiment, which comprises the following steps of: according to experimental design, selecting functional leaves, avoiding overripe leaves, over tender leaves, leaves damaged by diseases and insects and malformed leaves, and cleaning object leaves; evenly coating a thin layer of nail polish at a position to be observed, and natural drying or blowing to be nearly dry by using a washing ear ball; flatly and tightly pasting a transparent adhesive tape on a clean glass slide, using a dissecting needle or a clean blade for slowly guiding to prevent air bubbles, and tightly pasting the adhesive tape with the width being at least 2mm around the nail polish on the glass slide; and numbering, clean packaging, and taking back to the laboratory for microscopic observation and measurement. The method has the advantages of being capable of bulk sampling in field living leaves, having no damage to the leaves, fixed position recording stomatal conditions, observing daily change of the stomata of the leaves under different processing conditions, and the like, and can be used for the research and practice in the fields such as bioecology, plant physiology, plant germplasm resource, plant thremmatology, cultivation science, etc.

Description

technical field [0001] The invention relates to the technical field of plant physiology and ecology research, in particular to a technical method for studying plant stomatal characteristics and dynamic changes thereof without damage and positioning a large number of samples under field test living conditions. Background technique [0002] Leaf is the main organ for photosynthesis and transpiration in plants, and the CO required for photosynthesis in leaves 2 It enters the interior of the blade from the atmosphere through the stomata. At the same time, the water in the plant is lost from the stomata to the atmosphere through the transpiration of the leaves. Therefore, the shape, quantity and distribution of the stomata on the leaf epidermis are closely related to the physiological functions of the leaves. In addition, it is also closely related to the growth environment of the plant. [0003] In the evaluation of germplasm resources, stomata, as one of the important botanic...

AI Technical Summary

Problems solved by technology

The desiliconization scraping method uses less equipment and reagents, is simple and easy to operate, has clear images, and accurate observation results; but the disadvantage is that the leaves are carried out under in vitro conditions, and the stomata seen are closed, so it cannot It is used for the study of stomatal opening and closing status or opening variation law, which has become the fatal shortcoming of this method

[0016] The above methods of observing stomata have certain advantages and operability. Some methods are simple, inexpensive, and easy to operate, but the observed stomata are often closed; some have high accuracy but are complicated to operate or require precision instruments.

These methods have certain limitations, such as being affected by the texture of the leaf, the leaf surface hairs or epidermal appendages, and the effect of film production, and more importantly, the field sampling and dynamic characteristics of stomatal characteristics are limited.

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