Sampling method for non-damage fixed position observation of plant stomata in field experiment

A field-tested, non-invasive technology, applied in the field of plant physiology and ecology research, which can solve the problems of complex operation, limited dynamic characteristics, inability to use stomatal opening and closing conditions or the changing law of opening degree, etc.

Inactive Publication Date: 2009-10-14
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The desiliconization scraping method uses less equipment and reagents, is simple and easy to operate, has clear images, and accurate observation results; but the disadvantage is that the leaves are carried out under in vitro conditions, and the stomata seen are closed, so it cannot It is used for the study of stomatal opening and closing status or opening variation law, which has become the fatal shortcoming of this method
[0016] The above methods of observing stomata have certain advanta

Method used

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  • Sampling method for non-damage fixed position observation of plant stomata in field experiment
  • Sampling method for non-damage fixed position observation of plant stomata in field experiment
  • Sampling method for non-damage fixed position observation of plant stomata in field experiment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 Observation of Stomata of Munage Grape

[0042] In August 2008, in the grape corridor of Dahan Village, Tuohula Township, Wensu County, Aksu Prefecture, Xinjiang, in the study of the effects of soil and foliar fertilization on the photosynthetic characteristics of Munage grapes, the stomata of Munage grape leaves were sampled. . The method is to select leaves with normal photosynthetic function on the sunny side (usually the third or fourth leaf from the base of the branches) according to different soil fertilization treatments and foliage spray treatments. Since the test site is on the roadside of the village, there is a lot of dust The dust on the surface of the blade is cleaned by blowing, wiping, brushing and other means. On the part near the main vein of the leaf, carefully apply the nail polish film, the area is less than 1cm 2 , use mouth blowing to speed up drying, use 15mm×15mm transparent plastic tape to remove the dried nail polish film, quickly ...

Embodiment 2

[0043] Example 2 Observation of Stomata of Red Globe Grape

[0044] In August 2008, in Hongqipo Farm, Aksu District, Xinjiang, in the study of the effect of soil and foliar fertilization on the photosynthetic characteristics of Red Globe Grape, the stomatal sampling of Red Globe Grape leaves was studied. The method is to select the leaves with normal photosynthetic function on the sunny side (the third or fourth leaf from the base of the branch) according to different soil fertilization treatments and spray fertilizer treatments on the leaves, first blow and wipe the surface of the leaves, and place them near the main veins of the leaves. Parts, carefully apply nail polish film, the area is 5mm×5mm, use ear washing ball to blow air to speed up drying, use transparent plastic tape (15mm×15mm) to remove the dried nail polish film, quickly paste it on the glass slide, each The slides are all numbered, including the treatment name, time, etc., and more than 3 repeated samples are ...

Embodiment 3

[0045] Example 3 Observation on daily changes of stomata in Wensu red grapes

[0046] In August 2008, in Wensu County, Aksu Prefecture, Xinjiang, in the research on the selection of Wensu red grape varieties, the stomatal sampling research of Wensu red grape leaves was carried out, especially for the trophic variant lines with obvious different fruit morphology. Changes and other characteristics, in order to provide a basis for variety selection. The method is the same as in example 2, see the result Figure 3-5 , image 3 The left and right pictures are respectively 10:00 and 12:00 Beijing time; Figure 4 14:00 and 16:00 respectively; Figure 5 Stomatal characteristics of Wensu red grapes at 18:00 and 20:00 respectively.

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Abstract

The invention discloses a sampling method for non-damage fixed position observation of plant stomata in a field experiment, which comprises the following steps of: according to experimental design, selecting functional leaves, avoiding overripe leaves, over tender leaves, leaves damaged by diseases and insects and malformed leaves, and cleaning object leaves; evenly coating a thin layer of nail polish at a position to be observed, and natural drying or blowing to be nearly dry by using a washing ear ball; flatly and tightly pasting a transparent adhesive tape on a clean glass slide, using a dissecting needle or a clean blade for slowly guiding to prevent air bubbles, and tightly pasting the adhesive tape with the width being at least 2mm around the nail polish on the glass slide; and numbering, clean packaging, and taking back to the laboratory for microscopic observation and measurement. The method has the advantages of being capable of bulk sampling in field living leaves, having no damage to the leaves, fixed position recording stomatal conditions, observing daily change of the stomata of the leaves under different processing conditions, and the like, and can be used for the research and practice in the fields such as bioecology, plant physiology, plant germplasm resource, plant thremmatology, cultivation science, etc.

Description

technical field [0001] The invention relates to the technical field of plant physiology and ecology research, in particular to a technical method for studying plant stomatal characteristics and dynamic changes thereof without damage and positioning a large number of samples under field test living conditions. Background technique [0002] Leaf is the main organ for photosynthesis and transpiration in plants, and the CO required for photosynthesis in leaves 2 It enters the interior of the blade from the atmosphere through the stomata. At the same time, the water in the plant is lost from the stomata to the atmosphere through the transpiration of the leaves. Therefore, the shape, quantity and distribution of the stomata on the leaf epidermis are closely related to the physiological functions of the leaves. In addition, it is also closely related to the growth environment of the plant. [0003] In the evaluation of germplasm resources, stomata, as one of the important botanic...

Claims

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Application Information

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IPC IPC(8): G01N1/02G01N21/84
Inventor 张宗勤王跃进张朝红纪薇丁家华
Owner NORTHWEST A & F UNIV
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