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High-fat feed and application thereof in building animal model with non-alcoholic fatty liver

A high-fat feed, non-alcoholic technology, applied in applications, animal feed, animal feed, etc., can solve problems such as instability, long molding time, and difficulty in model TG hyperemia, and achieve easy control and simple operation Effect

Inactive Publication Date: 2011-06-29
ZHEJIANG ACAD OF MEDICAL SCI
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  • Abstract
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Problems solved by technology

[0005] The method of using SD rats as experimental animals to construct NAFLD animal models is the most commonly used method at present, but this method takes a long time to model, and the model TGemia is difficult or unstable; in addition, using SD rats as experimental animals, the model Stuck at the stage of the steatohepatitis model, so the model cannot be used to establish a systematic serological diagnostic index for clinical diagnosis of disease progression
The above defects limit the use of this method in the study of the etiology of NAFLD and drug evaluation.
[0006] Rats and other conventional experimental animals are fed with ordinary animal feed, and the model is only stagnant in the steatohepatitis model stage, and it is impossible to establish an animal model of non-alcoholic fatty liver fibrosis

Method used

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  • High-fat feed and application thereof in building animal model with non-alcoholic fatty liver
  • High-fat feed and application thereof in building animal model with non-alcoholic fatty liver
  • High-fat feed and application thereof in building animal model with non-alcoholic fatty liver

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Embodiment 1

[0031] Healthy male adult long-clawed gerbils were selected as experimental animals and randomly divided into model group and normal control group. The normal control group continuously ate common rat breeding diet (provided by Zhejiang Experimental Animal Feed Production Base) ad libitum. The model group continued to eat high-fat diet ad libitum to establish NAFLD model. The high-fat feed is made up of the following raw materials in mass percentage: 80.5% of common rat breeding feed (provided by Zhejiang Experimental Animal Feed Production Base), 10% of eggs, 7% of lard, 2% of cholesterol, No. 3 bile salt (Hangzhou Microbiological Reagent Co., Ltd.) 0.5%; the preparation method of high-fat feed is as follows: eggs (with shells) are mashed, mixed evenly with common rat breeding feed, lard, cholesterol and No. 3 bile salt, to obtain high-fat feed. At the end of the 2nd week, 6th week, and 16th week of free feeding, the experimental animals were sacrificed. The tissue in the m...

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Abstract

The invention discloses high-fat feed and application thereof in building an animal model with non-alcoholic fatty liver. The high-fat feed comprises the following raw materials in mass percent: 80.5% of rat breeding feed, 10% of egg, 7% of lard oil, 2% of cholesterol and 0.5% of No.3 bile salt. The model built in the invention can subsequently experience former three stages in the development ofthe clinical disease course of the non-alcoholic fatty liver: simple fatty liver, steato hepatitis and fibrosis. The model is used for studying the pathogenesis as well as the development mechanism of the non-alcoholic fatty liver, studying the prevention and treatment of the non-alcoholic fatty liver at different stages, and particularly for screening new medicines for preventing and treating fibrosis of the non-alcoholic fatty liver, screening measures for preventing and treating fibrosis of the non-alcoholic fatty liver and objectively evaluating the methods for preventing and treating thenon-alcoholic fatty liver at each stage.

Description

technical field [0001] The invention relates to the technical field of medical evaluation and detection, in particular to a high-fat feed and the application of the high-fat feed in constructing an animal model of non-alcoholic fatty liver. The constructed animal model of non-alcoholic fatty liver can be used to study non-alcoholic fatty liver Pathogenesis and development mechanism of non-alcoholic fatty liver and the prevention and treatment of non-alcoholic fatty liver fibrosis. Background technique [0002] Nonalcoholic fatty liver disease (NAFLD) is a clinicopathological syndrome characterized by fat accumulation and steatosis in hepatic parenchymal cells without a history of excessive alcohol consumption. According to the degree of liver histopathological changes, it can be divided into simple fatty liver, steatohepatitis, liver fibrosis and liver cirrhosis. Simple fatty liver only manifests as fatty degeneration of liver cells. Liver cell inflammation on the basis of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K10/20A23K20/142A23K20/158A23K20/168A23K50/50
Inventor 李巍石巧娟萨晓婴郭红刚楼琦卢领群
Owner ZHEJIANG ACAD OF MEDICAL SCI
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