Function of composition of Chinese date and rhizoma polygonati in improving hemoglobin content and oxidation resistance

A composition and anti-oxidation technology, applied in the direction of medical preparations containing active ingredients, drug combinations, applications, etc., can solve the problems of no research reports on jujube and sealwort, and few research objects

Inactive Publication Date: 2011-07-20
于英君 +3
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the past, sports, medicine, and nutrition have cooperated to develop some powerful nutritional products to improve sports performance, and most of them are chemical co...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: Experimental animal selection grouping

[0010] 20-22 g of clean-grade Kunming mice were selected, both male and female (provided by the Safety Evaluation Center of Heilongjiang University of Traditional Chinese Medicine). They were randomly divided into ten groups of blank control group, jujube and sealwort composition group, with 28 rats in each group, among which the jujube and sealwort composition group adopted an orthogonal experimental design.

Embodiment 2

[0011] Example 2: Detection of Hb content in mice

[0012] 1. Conventional methods for sample collection and processing

[0013] 2. Determination of hemoglobin: cyanide methemoglobin method

[0014] Principle: Hb interacts with sodium azide and ferricyanide to form ferric azide Hb, which is exactly the same as ferric cyanide Hb, and has a maximum absorption value at 540 or 546nm. Measure the absorbance value at 546nm wavelength and calculate its content.

[0015] Method: Enter the following parameters according to the kit instructions: wavelength 546nm, temperature 37 degrees, delay time 2 minutes, measurement time 2 minutes, standard value 100g / L, inhalation volume 1000ul. Set the reagent blank solution to zero, input the standard value of 100, inhale the standard solution for calibration, measure the sample tubes one by one and the result will be displayed.

[0016] Experimental results: the combination of jujube and sealwort has a significant effect on increasing the cont...

Embodiment 3

[0017] Embodiment three: the mensuration of succinate dehydrogenase:

[0018] Principle: Succinate dehydrogenase catalyzes the substrate reaction, FAD is the prosthetic group of the reaction, FAD is reduced to FADH, this reaction is coupled with the reduction of 2,6-DPIP, and the reduction rate of 2,6-DPIP can be measured Calculate the vitality of SDH.

[0019] Method: Use 721 spectrophotometer for detection, adjust to zero with distilled water, read the absorbance OD value after 5 seconds of reaction, and read the OD value again after one minute.

[0020] The experimental results show that the combination of jujube and Polygonatum has good antioxidant function. It has been reported that Polygonatum extract is rich in antioxidant components, which can effectively improve the defense system to protect mitochondria. This experiment measures the activity of SDH in mouse serum after an exhaustive exercise. SDH exists on the inner membrane of mitochondria in many organs, mainly ca...

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Abstract

The invention discloses a new composition of Chinese date and rhizoma polygonati, which is food as well as medicament and can improve the hemoglobin content and oxidation resistance and enhance the cell membrane repairing function. The preparation method of the composition is simple and easy to implement large-scale industrial production. The composition of Chinese date and rhizoma polygonati disclosed by the invention is obviously effective in improving the hemoglobin content and oxidation resistance, and has a good application prospect.

Description

technical field [0001] The invention discloses a novel composition of Chinese jujube and Polygonatum that is ready-to-eat and medicinal, and has the same source of medicine and food. The composition can increase hemoglobin content and antioxidant capacity, and improve cell membrane repairing function. It belongs to the field of functional food research and development. Background technique [0002] With the rapid development of the global economy, sports are getting more and more attention from all countries. It has gone beyond the original scope and ushered in a stage of rapid development. How to train more world-class elite athletes? How to improve the performance and fitness of athletes? This is a problem we urgently need to solve. However, high-intensity, high-volume physical training and physical training have led to the decline of athletes' athletic ability. It is important to choose reasonable recovery methods during the training process to help athletes supplemen...

Claims

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Application Information

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IPC IPC(8): A61K36/8969A23L1/29A61P39/06A23L33/00
Inventor 于英君李丽于水澜李和伟
Owner 于英君
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