Alfalfa molecule polymerization breeding method

A technology of alfalfa and seeds, applied in animal houses, applications, animal husbandry, etc., can solve problems such as difficulties, changes in genetic balance, and small possibilities, and achieve the effect of improving efficiency

Inactive Publication Date: 2011-08-10
SOUTHWEST UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to construct a multivalent plant expression vector. Therefore, it is usually necessary to carry out multiple transgenic operations to aggregate multiple genes into one plant. Due to the different insertion sites of the target gene, there are obvious differences in expression, and because the insertion of the target gene often changes the original genetic balance, most transgenic plants have poor vitality, and the possibility of obtaining normal plants with multiple genes is very small
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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Introduce the MDH and PEPC genes into two different alfalfa plants, respectively, to obtain MDH-transgenic plants and PEPC-transgenic plants;

[0017] (2) Plant the MDH-transgenic plants and the PEPC-transgenic plants together, pollinate them together during flowering, and harvest them together after the seeds mature to obtain the first generation of seeds; set up isolation during flowering and pollination to prevent foreign pollen from mixing and pollen from transgenic plants. run away

[0018] (3) Plant the obtained seeds in acidic soil, select 8 plants with good growth characteristics, and detect these 8 plants with molecular technology, and finally obtain 5 excellent individual plants that aggregate MDH and PEPC genes; Five excellent single plants with all the target genes were pollinated together, and the seeds were harvested after maturity, and a new line was bred.

Embodiment 2

[0020] (1) Introduce MDH, PEPC, and CS genes into three different alfalfa plants, and obtain MDH-transgenic plants, PEPC-transgenic plants, and CS-transgenic plants;

[0021] (2) Plant the MDH gene transgenic plants, PEPC gene transgenic plants and CS gene transgenic plants in one place, pollinate them together when they bloom, harvest them together after the seeds are mature, and obtain the first generation of seeds; plant the first generation of seeds in one place again, The same mixed pollination during flowering, mixed harvesting after the seeds mature, to obtain 2 generations of seeds; continue to plant the 2 generations of seeds in one place, also mixed pollination during flowering, mixed harvesting after the seeds mature, to obtain 3 generations of seeds; the above compound hybridization All are isolated to prevent the mixing of foreign pollen and pollen of transgenic plants;

[0022] (3) Plant the obtained third-generation seeds in acidic soil, select 13 plants with go...

Embodiment 3

[0024] (1) Introduce MDH, PEPC, and A-T genes into three different alfalfa plants, respectively, to obtain MDH-transgenic plants, PEPC-transgenic plants, and A-T-gene-transformed plants;

[0025] (2) Plant the MDH gene transgenic plants, PEPC gene transgenic plants and A-T gene transgenic plants in one place, pollinate them together when they bloom, harvest them together after the seeds mature, and obtain the first generation of seeds; plant the first generation of seeds in one place again, Mixed pollination is also performed during flowering, and the mixed seeds are harvested after the seeds are mature to obtain 2 generations of seeds; the above-mentioned compound hybridizations are all isolated to prevent the mixing of foreign pollen and the transgenic plant pollen escaping;

[0026] (3) Plant the obtained second-generation seeds in acidic soil, select 20 plants with good growth traits, and detect these 20 plants with molecular techniques. As a result, no excellent individual...

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PUM

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Abstract

The invention discloses an alfalfa molecule polymerization breeding method, comprising the following three steps of: obtaining a transgenic plant by using transgenic technology, and breeding seeds by using compound hybridization technique; selecting and polymerizing well single strains of all target genes by using the traditional selection method of adversity adaptability or phenotypic character and molecule testing technology; and breeding new species through mixture selection theory. In the method, the transgenic technology is combined with population improvement method, which is beneficial for breeding well species which are polymerized by multiple target genes; and the method is capable of enhancing alfalfa molecule polymerization breeding efficiency.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to an alfalfa molecular aggregation breeding method. Background technique [0002] Plant molecular breeding refers to the separation and extraction of the genetic information DNA molecules with the target traits of the donor plant or the target genes in them, and introduce them into the recipient cells (fertilized eggs, embryo cells, etc.) to be improved, so that they can be integrated, replicated, and expressed. And inheritance, and according to the need to select and breed excellent individuals with purpose traits, and cultivate new varieties with economic value. [0003] High yield, high quality, and stress resistance are one of the ultimate goals pursued by plant breeding. Finding functional genes related to high yield, high quality, and stress resistance is of great significance to breeding. In recent years, molecular breeding technology represented by molecular markers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K1/02
Inventor 玉永雄胡艳李佺马雪梅刘卢生甘智才
Owner SOUTHWEST UNIVERSITY
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