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Method for producing microbial polysaccharide fermentation liquor by using waste glucose mother liquor

A technology of glucose mother liquor and microbial polysaccharide, applied in bacteria and other directions, can solve the problems of non-compliance with industrial policies, high manufacturing cost, waste of resources, etc.

Active Publication Date: 2011-08-17
HEBEI XINHE BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned existing technologies have the disadvantages of wasting a large amount of resources, high manufacturing costs, and not conforming to national industrial policies.

Method used

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  • Method for producing microbial polysaccharide fermentation liquor by using waste glucose mother liquor
  • Method for producing microbial polysaccharide fermentation liquor by using waste glucose mother liquor

Examples

Experimental program
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Effect test

Embodiment 1

[0110] Sphingomonas CGMCCNo.1650 is used as the production strain, which is inserted into a medium containing carbon sources, necessary nitrogen sources and nutrients for fermentation; the fermentation process includes the following steps:

[0111] A, the pretreatment of waste glucose mother liquor: use the ozone of concentration 20,000 ppm to treat the glucose mother liquor diluted 5 times for 20 minutes, then add hydrogen peroxide, which accounts for 0.003% of the material mass and 2.5% by mass, for decolorization;

[0112] B. Expanded culture of strains;

[0113] C, the bacterial classification after expanding in the step B is that the inoculum size of 5% is fermented according to mass percent, and described fermentation medium is made up of the component of following mass percent:

[0114] Pretreated waste glucose mother liquor 4% peptone 0.18%

[0115] Soy flour 0.2% Potassium dihydrogen phosphate 0.15%

[0116] Defoamer 0.03% FeSO 4 10ppm

[0117] The remainder is...

Embodiment 2

[0154] Sphingomonas CGMCCNo.1650 is used as the production strain, which is inserted into a medium containing carbon sources, necessary nitrogen sources and nutrients for fermentation; the fermentation process includes the following steps:

[0155] A, the pretreatment of waste glucose mother liquor: use the ozone of concentration 30,000 ppm to treat the glucose mother liquor diluted 10 times for 30 minutes, then add hydrogen peroxide, which accounts for 0.005% of the mass of the material, and is 2.5% by mass for decolorization;

[0156] B. Expanded culture of strains;

[0157] C, the bacterial classification after expanding in the step B is that the inoculum size of 10% is fermented according to mass percent, and described fermentation medium is made up of the component of following mass percent:

[0158] Pretreated waste glucose mother liquor 6% peptone 0.25%

[0159] Soy flour 0.4% Potassium dihydrogen phosphate 0.3%

[0160] Defoamer 0.1% FeSO 4 15ppm

[0161] The rem...

Embodiment 3

[0197] Sphingomonas CGMCCNo.1650 is used as the production strain, which is inserted into a medium containing carbon sources, necessary nitrogen sources and nutrients for fermentation; the fermentation process includes the following steps:

[0198] A, the pretreatment of waste glucose mother liquor: use the ozone of concentration 25,000ppm to treat the glucose mother liquor diluted 8 for 25, then add hydrogen peroxide, which accounts for 0.004% of the mass of the material, and is 2.5% by mass for decolorization;

[0199] B. Expanded culture of strains;

[0200] C, the bacterial classification after expanding in the step B is that 7% inoculum size inserts fermentation medium and ferments according to mass percentage, and described fermentation medium is made up of the component of following mass percentage:

[0201] Pretreated waste glucose mother liquor 5 peptone 0.2%

[0202] Soy flour 0.3% Potassium dihydrogen phosphate 0.2%

[0203] Defoamer 0.07% FeSO 4 12ppm

[020...

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Abstract

The invention relates to microbial fermentation, in particular to a method for producing microbial polysaccharide fermentation liquor by using waste glucose mother liquor. In the method, sphingomonas CGMCC No,1650 is used as a productive strain, and the strain is subjected to expanding culture and then inoculated to a fermentation culture medium for fermentation, wherein the preprocessed waste glucose mother liquor is used as a carbon source for the fermentation culture medium. The invention overcomes the defects of huge raw material waste, high production cost, difficulty in environmental-protection and the like in the prior art. The method achieves the advantages of simple process, easiness for implementation, low manufacturing cost, better adaptability to an industrial policy in China in technological advancing direction, and the like on the premise of ensuring that product quality is equivalent to that of the traditional process.

Description

technical field [0001] The invention belongs to microbial fermentation, in particular to a method for producing microbial polysaccharide fermentation liquid by using waste glucose mother liquid. Background technique [0002] At present, the preparation of microbial polysaccharide-Sanzan Gum is to inoculate the production bacteria in the aseptic medium with starch as the main carbon source for fermentation. The patent number is 200610048338.X, and the invention name is a kind of sphingosine Bacteria and the method of producing microbial polysaccharides using the strain disclosed in the invention patents use Sphingomonas CGMCC No. Aerobic fermentation is carried out under certain temperature and pH conditions, and the end product of fermentation is extracted to obtain microbial polysaccharide-Sanzan gum. The above-mentioned prior art has the disadvantages of wasting a large amount of resources, high manufacturing cost, and not conforming to national industrial policies. Con...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 张禹张国佩张少华贾敬敏
Owner HEBEI XINHE BIOCHEM
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