Method for trace, fast and accurate detection of human erythrocyte antigen and antibodies in serum

Abstract

The invention relates to a method for trace, fast and accurate detection of human erythrocyte antigens and antibodies in serum. Tiny known antibodies for resisting erythrocyte antigens are allocated on a trace detection board by the principle of immunohematology, and tiny unknown erythrocytes to be detected are added for detecting the antigens at the surface of the erythrocyte membrane; or the erythrocytes of the tiny known erythrocyte antigens are added into the tiny unknown serum to be detected, so as to detect the antibody for resisting erythrocyte membrane surface antigens in human blood to be detected. The reaction characteristic is erythrocyte aggregation; if the aggregation exists, the reaction is a positive reaction, and otherwise the reaction is a negative reaction; and a centrifugal machine, a spectrophotometer, a microscope and a computer program are cooperated, so that the erythrocyte antigens and antibodies in serum can be detected fast, accurately and automatically. The use of the trace detection board kit prepared by the method in medical field can greatly improve the safety, efficiency and accuracy of clinical erythrocyte matching, reduce the detection expenses, and promote the development of clinical blood application.

Description

technical field [0001] The invention relates to a method for rapidly and accurately detecting human red blood cell antigens and antibodies in serum by using the principle of immunohematology, in particular to a trace, fast and accurate method for detecting human red blood cell antigens and antibodies in serum. Background technique [0002] In 1901, Kaul Landsteiner proved that when blood is transfused between groups of people, the different blood types of red blood cells can cause fever and jaundice in the transfused person, so he divided the blood types into A, B, and O types, and also explained in practical applications There will be no transfusion reactions between people whose blood types match. In 1902 A.Decastrello and A.Sturli discovered the AB blood type. The Rh blood type was discovered by Levine and Stetson in 1940, which is based on the presence or absence of this antigen on the red blood cell membrane. does not contain this antigen. It is now clear that more t...

AI Technical Summary

Problems solved by technology

The method of using glass slides is simple and fast, but it consumes a lot of reagents and is not very accurate; it consumes time and is inefficient; the test tube method is accurate, but it is time-consuming, consumes reagents, has low efficiency, and the results cannot be preserved; the gel method is accurate and the results can be preserved. But time-consuming, reagent-consuming, and low efficiency; the results of the check-board method can be saved, fast, and can be conveniently automated. The efficiency is high, but the reagents are expensive, the results cannot be saved, the operation is troublesome, it is not intuitive, and sometimes errors occur, and manual review is required.

Images