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Anti-helicobacter pylori bovine colostrum product

An anti-Helicobacter pylori and Helicobacter pylori technology, applied in the biological field, can solve the problems such as unsatisfactory treatment of Helicobacter pylori, unsatisfactory effect of Helicobacter pylori, easy generation of drug-resistant strains, etc. Simple and less side effects

Inactive Publication Date: 2011-09-14
江西英雄乳业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the treatment of Helicobacter pylori (HP) infection is not ideal
In the current routine treatment of HP, there are problems such as high treatment cost, large side effects, and easy to produce drug-resistant strains.
In recent years, it has been reported that bovine colostrum immunoglobulin has a certain therapeutic effect on HP infection, but the effect has not met the requirements for the actual treatment of Helicobacter pylori

Method used

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  • Anti-helicobacter pylori bovine colostrum product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: The bovine colostrum product against Helicobacter pylori is realized through the following steps: preparation of Helicobacter pylori antigen, immunization of Helicobacter pylori antigen, collection of immunized milk, and preparation of immunized milk into dairy products.

[0043] Wherein the preparation of Helicobacter pylori antigen has the following steps:

[0044] (1) Cultivation of strains

[0045] Take out a tube of Helicobacter pylori international standard strain NCTC 11637 Bacteria, add 1 drop of bacteria solution to the solid culture plate, spread evenly with a spreader stick, in 5% O 2 , 10%CO 2 , humidity 95% and temperature 37 o C culture conditions for 4 days.

[0046] The composition of the medium is tryptone 12g, yeast extract 3g, polyvalent peptone 15g, glucose 2g, NaCl 6g, agar 12g, soluble starch 20g, H 2 O1500mL, 12 grams of sheep blood.

[0047] (2) Strain passage

[0048] Scrape bacteria directly from the cultured plate with a co...

Embodiment 2

[0060] Example 2: The bovine colostrum product against Helicobacter pylori is realized through the following steps: preparation of Helicobacter pylori antigen, immunization of Helicobacter pylori antigen, collection of immunized milk, and preparation of immunized milk into dairy products.

[0061] Wherein the preparation of Helicobacter pylori antigen has the following steps:

[0062] (1) Cultivation of strains

[0063] Take out a tube of Helicobacter pylori international standard strain NCTC 11637 Bacteria, add 1 drop of bacteria solution to the solid culture plate, spread evenly with a spreader stick, in 5% O 2 , 10%CO 2 , humidity 95% and temperature 37 o C culture conditions for 3 days.

[0064] The composition of the medium is tryptone 12g, yeast extract 3g, polyvalent peptone 15g, glucose 2g, NaCl 6g, agar 12g, soluble starch 18g, H 2 O1500mL, 12 grams of sheep blood.

[0065] (2) Strain passage

[0066] Scrape bacteria directly from the cultured plate with a co...

Embodiment 3

[0071] Example 3: The bovine colostrum product against Helicobacter pylori is realized through the following steps: preparation of Helicobacter pylori antigen, immunization of Helicobacter pylori antigen, collection of immunized milk, and preparation of immunized milk into dairy products.

[0072] Wherein the preparation of Helicobacter pylori antigen has the following steps:

[0073] 1) Cultivation of strains

[0074] Take out a tube of Helicobacter pylori international standard strain NCTC 11637 Bacteria, add 1 drop of bacteria solution to the solid culture plate, spread evenly with a spreader stick, in 5% O 2 , 10%CO 2 , humidity 95% and temperature 37 o C culture conditions for 4 days.

[0075] The composition of the medium is tryptone 12g, yeast extract 3g, polyvalent peptone 15g, glucose 2g, NaCl 6g, agar 12g, soluble starch 25g, H 2 O1500mL, 12 grams of sheep blood.

[0076] (2) Strain passage

[0077] Scrape bacteria directly from the cultured plate with a coa...

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Abstract

The invention relates to an anti-helicobacter pylori bovine colostrum product which is realized by the following steps of: preparing helicobacter pylori antigens; immunizing the helicobacter pylori antigens; collecting immune milk; and preparing the immune milk into a milk product. The anti-helicobacter pylori bovine colostrum product prepared in the invention not only has the good effects of preventing and treating diseases caused by helicobacter pylori infection, but also has the advantages of good specificity, fewer side effects, difficulty in generating drug-resistant strains, simple and convenient preparation method, easiness for industrialization and the like.

Description

technical field [0001] The invention relates to a bovine colostrum product against Helicobacter pylori and belongs to the field of biotechnology. Background technique [0002] Helicobacter pylori (HP) is a common pathogenic microorganism in humans. HP is not only the main pathogen of peptic ulcer and chronic gastritis, but also closely related to the occurrence of gastric cancer, which seriously endangers human health. HP infection is distributed globally, and the infection rate increases with age, especially in developing countries. About 40% of people in developed countries and 80% of people in developing countries have been infected with HP in adulthood. my country is a country with high HP infection rate and high incidence rate of gastric cancer in the world. [0003] Immune milk refers to selectively inoculating mammals (mainly cows, goats, etc.) with some bacteria, viruses or other foreign antigens (Antigen) that can cause human or animal diseases, and stimulates the...

Claims

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Application Information

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IPC IPC(8): A23C9/20
Inventor 江小芬
Owner 江西英雄乳业股份有限公司
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