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Method for extracting carthamin from sculellaria barbata

A technology of safflower and Scutellaria barbata, applied in the direction of organic chemistry, can solve the problems of large raw material loss and long preparation cycle, and achieve the effect of less sample loss, large processing capacity and simple elution solvent

Inactive Publication Date: 2011-09-14
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method has a long preparation period and a large loss of raw materials

Method used

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  • Method for extracting carthamin from sculellaria barbata

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Take 1kg of the whole herb of Scutellaria barbata, crush it, add 4L of 40% ethanol to reflux and extract 4 times, each time for 1.5h, separate the solid and liquid to obtain the extract, concentrate until it has no alcohol smell, take 350g of 80-mesh polyamide resin, put it on a column, and put it on the concentrated solution The column was eluted with 8BV of 30% ethanol containing 1% acetic acid, and the solvent was recovered from the eluent to obtain the crude extract of safflower; n-butanol, acetic acid, and water were placed in a liquid separation according to a volume ratio of 3:1:4. Mix in the funnel, let stand to separate layers, separate the upper and lower phases, take the upper phase as the stationary phase, and the lower phase as the mobile phase, dissolve the crude safflower extract in the lower phase, turn on the preparative high-speed countercurrent chromatograph, and separate the stationary phase Pump the chromatograph to the full, then pump the mobile pha...

Embodiment 2

[0022] Take 5kg of the whole herb of Scutellaria barbata, crush it, add 10L of 20% ethanol to reflux for extraction for 0.5h, separate the solid and liquid to obtain the extract, concentrate until it has no alcohol smell, take 5kg of 120-mesh polyamide resin, pack it into a column, put the concentrated solution on the column, and use 4BV 50% ethanol containing 1% acetic acid was eluted, and the solvent was recovered from the eluent to obtain the crude extract of safflower; n-butanol, acetic acid, and water were mixed in a separatory funnel in a volume ratio of 7:3:10, Stand for stratification, separate the upper and lower phases, take the upper phase as the stationary phase, and the lower phase as the mobile phase, dissolve the crude safflower extract in the lower phase, turn on the preparative high-speed countercurrent chromatograph, and pump the stationary phase to the top of the chromatograph, Then pump the mobile phase with a flow rate of 3.5ml / min, and continuously inject ...

Embodiment 3

[0024] Take 10kg of the whole herb of Scutellaria barbata, crush it, add 80L of 30% ethanol to reflux and extract twice, each time for 1 hour, separate the solid and liquid to obtain the extract, concentrate until it has no alcohol smell, take 6kg of 80 mesh polyamide resin, pack it into a column, and put the concentrated solution on the column , eluted with 45% ethanol containing 1% acetic acid of 6BV, and the solvent was recovered from the eluent to obtain the crude extract of safflower; take n-butanol, acetic acid, and water in a separatory funnel in a volume ratio of 6:2:7 Mix evenly in the middle, let stand to separate layers, separate the upper and lower phases, take the upper phase as the stationary phase, and the lower phase as the mobile phase, dissolve the crude safflower extract in the lower phase, turn on the preparative high-speed countercurrent chromatograph, and pump the stationary phase Fill the chromatograph, then pump the mobile phase with a flow rate of 10ml / ...

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Abstract

The invention provides a method for extracting carthamin from sculellaria barbata. The method comprises the following steps: smashing the whole sculellaria barbata; refluxing with low-concentration alcohol for extraction; concentrating extract until alcohol is exhausted; absorbing on a polyamide resin column; eluting with acid and alcohol; recovering a solvent from eluent; purifying with a high-speed counter-current chromatography; collecting effluent; concentrating; and drying at low temperature so as to obtain the high-purity carthamin. The method is efficient, has no pollution and the product has high purity.

Description

Technical field: [0001] The invention belongs to the field of separation of natural products, and in particular relates to a method for extracting safflower from Scutellaria barbata by applying high-speed countercurrent chromatography technology. Background technique: [0002] Banzhilian (Scutellaria babarta D.Don.), also known as Scutellaria babarta D.Don., also known as narrow-leaved Hanxin grass, toothbrush grass, etc., its name was first seen in "Surgery Authentic", and it is the whole herb of the Labiatae plant Banzhilian. Cold in nature, it returns to the lung, liver and kidney meridians, and has the functions of clearing heat and detoxifying, dispelling blood stasis to stop bleeding, diuresis and swelling, and is a commonly used traditional Chinese medicine. Scutellaria barbata contains alkaloids, phenols, steroids, tannins, flavonoids and other active ingredients, among which flavonoids are the most abundant in this plant and are the main active ingredients. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/32C07D311/40
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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