Method for introducing mutated gene, gene having mutation introduced therein, cassette for introducing mutation, vector for introducing mutation, and knock-in non-human mammal

Abstract

Disclosed is a method for introducing a mutation into a gene, which comprises the following steps: a homologous recombination step of carrying out the homologous recombination between a target gene into which the mutation is to be introduced and a target recombinant vector, thereby substituting an exon in the target gene into which the mutation is to be introduced by a target DNA sequence in the target recombinant vector; and a mutation introduction step of carrying out the specific recombination between the target DNA sequence in the resulting recombined target gene and a mutation introduction cassette of a mutation introduction vector carrying a mutated DNA sequence containing a mutated exon by the intervening action of Cre recombinase to substitute the target DNA sequence by the mutated DNA in the mutation introduction cassette, thereby producing a mutation-introduced gene into which the mutant DNA sequence has been introduced. The method enables the production of a knock-in non-human animal, such as a knock-in mouse, which carries the mutation-introduced gene.

Description

technical field

[0001] The invention relates to a mutation gene introduction method, a mutation introduction gene, a mutation introduction cassette, a mutation introduction carrier and a gene knock-in non-human mammal. More specifically, the present invention relates to a method for introducing a mutant gene, a gene for introducing a mutation and a method for preparing the same, a cassette for introducing a mutation, and a carrier for introducing a mutation, and Knock-in of non-human mammals with mutations introduced into genes. Background technique

[0002] In organisms, it is not an exaggeration to say that genes, a "design map" that holds genetic information, are used to control all the functions used to maintain their life. The entire genetic information of these genes is controlled by a combination of base pairs consisting of 4 bases called DNA. If the combination of the base pair changes abnormally for some reason, resulting in a gene mutation, the genetic informatio...

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