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Method for extracting cottonseed oligosaccharide and cottonseed protein oligopeptide from cottonseed meal

A cottonseed protein and cottonseed meal technology is applied in the field of extracting cottonseed oligosaccharides and cottonseed protein oligopeptides from cottonseed meal, and can solve problems such as being unsuitable for large-scale industrial production, not too high in efficiency and purity, and complicated in methods. , to achieve the effect of high food safety, simple process and water saving

Inactive Publication Date: 2013-04-10
武汉普赛特膜技术循环利用有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN 101785527A "A method for preparing dephenolized cottonseed protein and raffinose" uses an organic solvent method for the extraction of protein and sugar in cottonseed meal. The gossypol is completely removed, but it also removes the organic solvent into protein and sugar, and its method is more cumbersome, complicated, and the efficiency and purity are not too high, so it is not suitable for large-scale industrial production

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0032] Take 10kg of cottonseed meal and crush it with a particle size of 80 mesh; add 150kg of water, stir for 1 hour at a temperature of 30°C, and centrifuge to obtain the supernatant and filter residue; the supernatant is removed through a microfiltration membrane, and the operating condition is pressure 0.2MPa, temperature 40°C, flow rate 90L / min, to obtain concentrate and filtrate; the filtrate is then concentrated by nanofiltration membrane, the operating conditions are pressure 1.5MPa, temperature 40°C, flow rate 20L / min; Spray-dry the concentrated solution to obtain 0.62kg of oligosaccharides (white powder); and add 150kg of water to the concentrated solution of the slag after water extraction and the microfiltration membrane to dissolve in alkali, adjust the pH to 10, and the temperature to 80°C, stirring 1h; carry out enzymolysis after alkali dissolution, adjust the pH to 7.5, the temperature is 55°C, add 500g of alkaline protease, and enzymolysis for 2h; after the enz...

Embodiment 2

[0034] Take 10kg of cottonseed meal, crush it, and the particle size is 80 mesh; add 150kg of water, stir for 1 hour at a temperature of 40°C, and centrifuge to obtain the supernatant and filter residue; the supernatant is removed through a microfiltration membrane, and the operating condition is pressure The concentration is 0.25MPa, the temperature is 50°C, and the flow rate is 90L / min to obtain the concentrate and the filtrate; the filtrate is then concentrated with a nanofiltration membrane, and the operating conditions are as follows: a pressure of 2MPa, a temperature of 50°C, and a flow rate of 20L / min; The concentrated solution was spray-dried to obtain 0.625kg of oligosaccharides (white powder); and the slag after water extraction and the concentrated solution of the microfiltration membrane were added with 150kg of water for alkali dissolution, and the pH was adjusted to 11, the temperature was 85°C, and stirred for 2h ;After the alkali dissolution, carry out enzymatic...

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Abstract

The invention relates to a method for extracting cottonseed oligosaccharide and cottonseed protein oligopeptide from cottonseed meal. The cottonseed meal of which the grease is extracted by a low-temperature desolventizing technology is used as a raw material, the content of gossypol in the cottonseed meal is less than or equal to 60mg / kg, and all materials are added in an amount based on the weight of the cottonseed meal. The method comprises the following step of: grinding the cottonseed meal, performing intermediate temperate water extraction, performing centrifugal separation, removing impurities from supernatant fluid by using a micro filter membrane, concentrating by using a nanofiltration membrane, and performing spray drying to obtain the cottonseed oligosaccharide; and performingalkaline dissolution on residues obtained after the water extraction and the centrifugal separation and micro filter membrane concentrated solution, performing enzymolysis, decoloration, enzyme deactivation and centrifugal separation, removing the impurities from the supernatant fluid by using the micro filter membrane, concentrating by using the nanofiltration membrane, and performing spray drying on the concentrated solution to obtain the cottonseed protein oligopeptide. In the invention, cottonseed meal resources are fully utilized, a product with a high added value is produced, a process is simple and practical, and the method is suitable for industrialization production; and the obtained cottonseed oligosaccharide has the purity of 95 percent, the content of protein in the cottonseedprotein oligopeptide is more than 90 percent, and the molecular weight of the cottonseed protein oligopeptide is mainly distributed in the range of between 200 and 1,000Dal.

Description

technical field [0001] The invention relates to a production method for intensively processing cottonseed by using biotechnology, and mainly relates to a method for preparing cottonseed oligosaccharides and cottonseed protein oligopeptides by using cottonseed meal produced after oil and fat extraction by a low-temperature precipitation method as raw materials. Background technique [0002] my country is a large cotton-producing country, accounting for more than 60% of the world's total cotton output. In 2008, the cotton output was 20.526 million tons, and 11.49 million tons of cottonseed were produced. After oil extraction, 6.43 million tons of cottonseed meal were produced, which contained about 3.2 million tons of protein. About 300,000 tons of polysaccharides. For many years, about 5% of cottonseed meal after cottonseed oil is used as animal feed, and more than 95% is used as fertilizer, wasting a lot of protein and oligosaccharide resources. [0003] Cottonseed meal cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12P21/06C07K4/10C07K1/34C07K1/14
Inventor 万端极吴正奇徐国念吕景炎李猷毛波万莹王旭
Owner 武汉普赛特膜技术循环利用有限公司
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