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Preparation method of gynostemma pentaphylla seed oil containing gama-linolenic acid

A technology of Gynostemma pentaphyllum and linolenic acid, which is applied in the direction of oil/fat refining and fat production, can solve the problems of low γ-linolenic acid, insufficient raw materials, shortage of raw materials of γ-linolenic acid, etc. simple method effect

Inactive Publication Date: 2012-12-19
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In foreign countries, the seeds of plants such as evening primrose and blackcurrant are used as raw materials to extract γ-linolenic acid. Due to the shortage of raw materials, the output is extremely limited; domestically, evening primrose is mainly used as raw material, but the wild resources of evening primrose are decreasing year by year, and artificial The planting area is small, and the content of gamma-linolenic acid in evening primrose is low, only 8% to 10%.
Therefore, the extraction of γ-linolenic acid at home and abroad is facing the problem of shortage of raw materials

Method used

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  • Preparation method of gynostemma pentaphylla seed oil containing gama-linolenic acid
  • Preparation method of gynostemma pentaphylla seed oil containing gama-linolenic acid
  • Preparation method of gynostemma pentaphylla seed oil containing gama-linolenic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Extraction

[0023] Take 100g of Gynostemma pentaphyllum seeds, dry and crush them into 65-mesh fine powder, place them in a Soxhlet extractor, add 450g of petroleum ether, extract at 70-75°C for 6 hours, and then rotary evaporate at 65°C to distill off the petroleum ether to obtain Gynostemma pentaphyllum seed oil 32g.

[0024] 2. Saponification and acidolysis

[0025] Take 25g of Gynostemma pentaphyllum seed oil, put it in a flask, add 11.25g of saponification solution, blow in nitrogen gas and stir, reflux at 40-45°C for 1 hour, pour out the mixed solution, adjust the pH value to 2.0 with 1mol / L hydrochloric acid, separate The oil phase was washed three times with hot water at 40°C to obtain Gynostemma pentaphyllum seed oil containing γ-linolenic acid, and the content of γ-linolenic acid in the Gynostemma pentaphyllum seed oil was 49.43%.

[0026] The above saponified liquid is a mixed solution of sodium hydroxide, distilled water and ethanol with a mass ratio o...

Embodiment 2

[0030] 1. Extraction

[0031] Take 100g of Gynostemma pentaphyllum seeds, dry and crush them into 65-mesh fine powder, place them in a Soxhlet extractor, add 500g of petroleum ether, extract at 70-75°C for 8 hours, and then rotary evaporate at 65°C to distill off the petroleum ether to obtain Gynostemma pentaphyllum seed oil 35g.

[0032] 2. Saponification and acidolysis

[0033] Take 25g of Gynostemma pentaphyllum seed oil, put it in a flask, add 25g of saponification solution, blow in nitrogen gas and stir, reflux at 40-45°C for 1 hour, pour out the mixed solution, adjust the pH value to 2.0 with 1mol / L hydrochloric acid, and separate the oil phase, washed three times with hot water at 40°C to obtain Gynostemma pentaphyllum seed oil containing γ-linolenic acid, the content of γ-linolenic acid in Gynostemma pentaphyllum seed oil was 49.43%.

[0034] The above saponified liquid is a mixed solution of sodium hydroxide, distilled water and ethanol with a mass ratio of 1:5.6:7....

Embodiment 3

[0038] 1. Extraction

[0039] Take 100g of Gynostemma pentaphyllum seeds, dry and crush them into 65-mesh fine powder, place them in a Soxhlet extractor, add 100g of petroleum ether, extract at 70-75°C for 7 hours, and then rotary evaporate at 65°C to distill off the petroleum ether to obtain Gynostemma pentaphyllum seed oil 30g.

[0040] 2. Saponification and acidolysis

[0041] Take 25g of Gynostemma pentaphyllum seed oil, put it in a flask, add 5g of saponification solution, blow in nitrogen gas and stir, reflux at 40-45°C for 1 hour, pour out the mixed solution, adjust the pH value to 2.0 with 1mol / L hydrochloric acid, and separate the oil phase, washed three times with hot water at 40°C to obtain Gynostemma pentaphyllum seed oil containing γ-linolenic acid, the content of γ-linolenic acid in Gynostemma pentaphyllum seed oil was 40.42%.

[0042] The above saponified liquid is a mixed solution of sodium hydroxide, distilled water and ethanol with a mass ratio of 1:5.6:7. ...

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Abstract

The invention discloses a preparation method of gynostemma pentaphylla seed oil containing gama-linolenic acid. The preparation method comprises the steps of: extracting gynostemma pentaphylla seed oil from gynostemma pentaphylla seeds with petroleum ether as an extracting agent; and carrying out saponification acidolysis and urea adduction upon the gynostemma pentaphylla seed oil, such that purified gynostemma pentaphylla seed oil containing gama-linolenic acid is obtained. As an experiment result, with the method provided by the present invention, the oil yielding rate of gynostemma pentaphylla seed oil from gynostemma pentaphylla seeds reaches about 30%, and the gama-linolenic acid content of the obtained gynostemma pentaphylla seed oil is 61.72% to 62.84%. The raw materials of the present invention are easy to obtain, and the preparation method is simple. The gama-linolenic acid content of the obtained gynostemma pentaphylla seed oil is high, and the preparation method can be applied in large-scale industrial productions.

Description

technical field [0001] The invention belongs to the technical field of straight-chain carboxylic acids containing 18 carbon atoms, in particular to a method for extracting gynostemma seed oil with high gamma-linolenic acid content from gynostemma seeds. Background technique [0002] γ-linolenic acid is an essential fatty acid to maintain the normal functions of the human body. It can be metabolized in the body to form dihomo-γ-linolenic acid or arachidonic acid, and then converted into prostaglandins and leukotrienes. A large number of studies and experiments have proved that γ-linolenic acid has a wide range of pharmacological effects, it has antibacterial, anti-tumor, anti-inflammatory, anti-HIV infection, inhibits platelet aggregation and thromboxane A 2 It can reduce plasma total cholesterol, triglyceride and low-density lipoprotein, increase high-density lipoprotein content, lower blood pressure, blood lipid, cholesterol and X-ray radiotherapy sensitization. [0003] γ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B3/00
Inventor 肖娅萍姜东亮马彩霞
Owner SHAANXI NORMAL UNIV