Juvenile pomfret gonad fixing and obtaining method

An acquisition method and juvenile fish technology are applied in the field of gonad fixation and acquisition of juvenile pomfret fish, which can solve the problems of difficult identification, small gonad tissue, and difficulty in stripping, and achieve the effects of easy acquisition, simple process and wide applicability.

Inactive Publication Date: 2013-03-20
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For pomfret adult fish, the gonads are relatively large and easy to find, so the process of obtaining and fixing them is relatively easy; however, for pomfret juveniles, due to their small size and small gonad tissue, some It is even only in the shape of a thin thread, which is not only difficult to identify during dissection, but also difficult to completely peel off

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Take fresh juvenile pomfret carcasses (4.5cm in total length), measure and number their biological data;

[0016] 2) Place the carcasses of fresh pomfret juveniles on the operating table, cut off the abdominal epidermis from the anus to the front with scissors, and then put the whole pomfret into Born's fixative for 24 hours;

[0017] 3) After 24 hours of fixation, the juvenile carcasses of pomfret were taken out of the Bones fixative solution and placed under a dissecting microscope, the epidermis above the abdomen was subtracted, and the rectum connected to the kidney and the upper end of the anus was found behind the gastrointestinal viscera mass. The connected pomfret gonad tissue that is fixed as yellow filaments is completely peeled off with tweezers;

[0018] 4) The stripped pomfret gonad tissue was fixed in 70% ethanol for a long time, to be used for tissue sectioning.

Embodiment 2

[0020] 1) Take the juvenile pomfret carcass (full length 7.5cm), measure its biological data and number it;

[0021] 2) Place fresh pomfret juvenile carcasses on the operating table, cut off the abdominal epidermis from the anus to the front with scissors, and then use a scalpel to cut off the head, tail and back muscles of the pomfret to leave the abdominal tissue, and then With the front end of the kidney and anus as the boundary, the first half of the abdomen was excised, and the rest of the second half of the abdomen was fixed in Bones' fixative for 24 hours;

[0022] 3) After 24 hours of fixation, the juvenile fresh pomfret carcasses were taken out of the Bones fixative solution and placed under a dissecting microscope, the epidermis above the abdomen was subtracted, and the gastrointestinal viscera mass was found, and the kidney and anus were found behind it. The pomfret gonad tissue connected to the rectum at the upper end is fixed as a light yellow thin line, and it is...

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PUM

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Abstract

The invention relates to a juvenile pomfret gonad fixing and obtaining method including the steps as follows: firstly, determining fresh dead juvenile pomfret, cutting the belly epidermis of the fresh dead juvenile pomfret to open, cutting away partial tissues and organs which do not contain gonad, then fixing the part containing gonad with Bouin fixative, at last, taking out the fixed tissue block and finding the gonad tissue of the pomfret behind the stomach intestine visceral mass under an anatomical lens. The juvenile pomfret gonad fixing and obtaining method can be used for sectioning inthe gonad histology of the juvenile pomfret and also applicable to research on the gonad histology of other aquatic fishes; and the juvenile pomfret gonad fixing and obtaining method has broad applicability.

Description

technical field [0001] The invention belongs to the technical field of fish histology research, and in particular relates to a method for fixing and obtaining gonads of juvenile pomfret fish. Background technique [0002] The gonad of fish is an important organ in the reproductive system. The cytological characteristics and changes of fish gonad tissue can be studied from the microscopic level through gonad tissue section technology, and the process and law of its occurrence, development and maturation can be clarified. Therefore, the histological study of fish gonads is of great significance in fish reproductive biology, and has been widely used in various fish researches. [0003] For pomfret adult fish, the gonads are relatively large and easy to find, so the process of obtaining and fixing them is relatively easy; however, for pomfret juveniles, due to their small size and small gonad tissue, some It is even only in the shape of a thin thread, which is not only difficul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 孙鹏尹飞施兆鸿彭士明
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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