Four-gradient elution analysis method of glycated hemoglobin analyzer

Abstract

The invention relates to a four-gradient elution analysis method of a glycated hemoglobin analyzer, which is used for detecting concentration of glycated hemoglobin via ionic exchange chromatography. The method comprises the following steps of: setting four kinds of eluent with fixed concentration; respectively using the four kinds of eluent to elute cation exchange resin having an absorbed sample to be detected; and performing chromatographic analysis on the eluted liquid to determine the content of the eluted ingredients, thereby determining a blood glucose concentration, wherein the four kinds of eluent are respectively as follows: an eluent A for washing and balancing the cation exchange resin, an eluent B for eluting other subfractions including HbA1a and HbA1b of the hemoglobin, an eluent C for eluting HbA1c subfraction of the glycated hemoglobin, and an eluent D for eluting an non-glycated hemoglobin and regenerated cation exchange resin. The method provided by the invention can accurately detect the concentration of the glycated hemoglobin, simplify structures of detection devices and detection programs, improve operational reliability of devices, decrease cost on devices and reduce cross contamination of the eluent.

Description

technical field [0001] The invention relates to a four-gradient elution analysis method of a glycated hemoglobin analyzer for detecting the concentration of glycated hemoglobin by ion exchange chromatography. Background technique [0002] The concentration of glycosylated hemoglobin in human blood is an important clinical indicator for the diagnosis of diabetes. Normal hemoglobin (Hb) will form various subcomponents after being glycosylated, but the most important subcomponent is HbA 1c , its structure is stable after its formation, and it has a life cycle of 90 to 120 days. By detecting this index, it can reflect the average blood sugar level of a diabetic patient for three months. Therefore, clinically, this indicator is generally used as an important basis for the screening, diagnosis and treatment of diabetic patients. [0003] The current methods for the determination of glycosylated hemoglobin include ion-exchange chromatography, electrophoresis, immunoturbidimetry ...

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Problems solved by technology

However, due to the use of eluents with different concentrations in the machine, it will inevitably increase the complexity of the instrument, increa

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