Method for screening candidate genes related to litter size of sow by using proteomics technology

A technology of candidate genes and litter size, applied in biochemical equipment and methods, microbe measurement/inspection, material inspection products, etc., can solve the problem of low screening efficiency

Inactive Publication Date: 2011-11-02
WUHAN UNIV
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Problems solved by technology

[0009] The purpose of the present invention is to provide a method for screening candidate genes related to pig litter size, in order to overcome the problem of low screening efficiency in the prior art

Method used

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  • Method for screening candidate genes related to litter size of sow by using proteomics technology
  • Method for screening candidate genes related to litter size of sow by using proteomics technology
  • Method for screening candidate genes related to litter size of sow by using proteomics technology

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Embodiment 1

[0040] Example 1 Screening of pig litter size related candidate genes

[0041] 1. Using 2D-DIGE technology to analyze the differential proteins in the ovaries of Meishan pigs, wild boars and their hybrid pigs

[0042] The ovaries of 2 Meishan pigs were taken, marked as A1 and A2; the ovaries of 2 wild boars (Sus Scrofa) and Meishan pigs were crossed, marked as B1 and B2; the ovaries of 2 Hubei wild boars were marked as C1, C2. Samples were frozen in liquid nitrogen immediately after collection and used in subsequent experimental sections. The above ovaries were all taken from Huagai breeding factory.

[0043] Each 300 mg tissue was cut into small pieces of 3 mm square on ice, washed 3 times with PBS, added DIGE lysate (DIGE lysate and protease inhibitor were mixed at a volume ratio of 50:1, 7M Urea, 2M Thiourea, 4 % CHAPS, Roche cooktail protease inhibitor) 1ml, homogenized with Dounce's homogenizer. Transfer to a centrifuge tube and ultrasonically break the cells (80W, 5 ...

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Abstract

The invention relates to a method for screening candidate genes related to litter size of sow by using a proteomics technology, and discloses a method for screening candidate genes related to litter size of the sow. With the present invention, a differential display is adopted for searching differential proteins in ovaries of specific heavy producing pig breeds of Meishan pigs in China and sus scrofa with low litter size, in particular, over-expression genes in the ovaries of the Meishan pigs, to quickly identify the genes related to the litter size of the sow a large scale. Compared to the previous method for screening the candidate genes, the method provided by the present invention is quick and exact; the method can be combined with a transgenic animal technology so as to provide motive power for breed of new transgenic variety and industrialization in China.

Description

technical field [0001] The invention relates to a method for screening genes, in particular to a method for screening candidate genes related to piglet farrowing. Background technique [0002] Domestic animals are generally domesticated from wild species after several years, and many of their important economic traits have been improved, such as growth speed, litter size, meat quality, and so on. [0003] Domestic pigs were domesticated approximately 9,000 years ago from wild boars, and similarly, many economically important traits have been improved through artificial selection over the past few thousand years, such as growth rate, meat quality, litter size, and disease resistance Wait. For example, Chinese Meishan pigs have a litter size of more than 18 pigs, while Chinese wild boars have a litter size of only about 8 pigs. [0004] Reproductive traits, especially litter size, are an important part of reducing the cost of pork production, so there is a lot of research in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68
Inventor 周荣家程汉华陈科
Owner WUHAN UNIV
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