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Method for seeking quality control index of raw material corn steep liquor for fermentation

A technology for controlling indicators and corn steep liquor, which is applied in the direction of measuring devices, material separation, and analysis of materials, etc., can solve the problems of inability to judge the quality of raw materials scientifically and accurately, and achieve good reproducibility and stability, high resolution and sensitivity, good repeatability

Active Publication Date: 2013-11-20
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These inspections cannot scientifically and accurately determine the quality of raw materials. Different batches of raw materials have a great impact on the potency of fermented products. Therefore, it is necessary to study its quality control index

Method used

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  • Method for seeking quality control index of raw material corn steep liquor for fermentation
  • Method for seeking quality control index of raw material corn steep liquor for fermentation
  • Method for seeking quality control index of raw material corn steep liquor for fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The method for seeking the quality control index of raw material corn steep liquor for fermentation comprises the following steps:

[0023] (1) Carry out chemical composition determination to 11 batches of different corn steep liquors:

[0024] ① Preparation of samples:

[0025] Put 1 g of uniform corn steep liquor sample in a centrifuge tube, centrifuge to collect the supernatant; dilute the obtained supernatant 20 times with ultrapure water, take 20 μL and put it in another centrifuge tube, add 30 μL of 0.040 mg / mL deuterium Marked methanolic succinic acid was used as internal standard. After freeze-drying, 50 μL of pyridine with a concentration of 20 mg / mL methoxyamine hydrochloride was added and dissolved in a 30° C. water bath for oximation reaction for 90 minutes. After the reaction was completed, 80 μL of N-methyl-N-trimethylsilane trifluoroacetamide was added and the silanization reaction was carried out in a water bath at 37°C for 30 minutes;

[0026] ②Gas c...

Embodiment 2

[0036] The method for seeking the quality control index of raw material corn steep liquor for fermentation comprises the following steps:

[0037] (1) Carry out chemical composition determination to 11 batches of different corn steep liquors:

[0038] ① Preparation of samples:

[0039] Put 2 g of uniform corn steep liquor sample in a centrifuge tube, centrifuge to collect the supernatant; dilute the obtained supernatant 40 times with ultrapure water, take 20 μL and put it in another centrifuge tube, add 40 μL of 0.040 mg / mL deuterium Marked methanolic succinic acid was used as internal standard. After lyophilization, 50 μL of 20 mg / mL methoxyamine hydrochloride in pyridine was added and dissolved in a water bath at 35° C. for oximation reaction for 120 min. After the reaction, add 70 μL of N-methyl-N-trimethylsilane trifluoroacetamide in a water bath at 35°C for silylation for 50 minutes;

[0040] ②Gas chromatography-time-of-flight mass spectrometer determination method:

...

Embodiment 3

[0047] The method for seeking the quality control index of raw material corn steep liquor for fermentation comprises the following steps:

[0048] (1) Carry out chemical composition determination to 11 batches of different corn steep liquors:

[0049] ① Preparation of samples:

[0050] Put 0.5 g uniform corn steep liquor sample in a centrifuge tube, centrifuge to collect the supernatant; dilute the obtained supernatant 20 times with ultrapure water, take 40 μL and put it in another centrifuge tube, add 30 μL of 0.040 mg / mL Deuterium-labeled succinic acid in methanol was used as the internal standard. After lyophilization, 70 μL of pyridine with a concentration of 20 mg / mL methoxyamine hydrochloride was added and dissolved in a water bath at 37° C. for oximation reaction for 60 min. After the reaction, add 100 μL of N-methyl-N-trimethylsilane trifluoroacetamide in a water bath at 40°C for silanization for 20 minutes;

[0051] ②Gas chromatography-time-of-flight mass spectrome...

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Abstract

The invention discloses a method for seeking a quality control index of raw material corn steep liquor for fermentation. The method comprises the following steps of: 1, measuring chemical constitutes of the corn steep liquor: (1) preparing a sample, and (2) adopting a gas chromatograph flight time mass spectrometer measurement method; 2, adopting a partial least squares discriminant analysis method: (1) standardizing and preprocessing data acquired by using the method 1, and (2) performing partial least squares discriminant analysis on the preprocessed data by using SIMCA-P11.5 software so as to obtain a mark map, a load map and a variable information processing (VIP) map which express the similarity and the difference of samples. By adoption of a high-flux and high-resolution detection technology, various chemical constitutes in the corn steep liquor can be detected; the method is simple; and due to verification of a plurality of batches of raw materials, the repetitiveness is high. The method is high in resolution and sensitivity and high in reproducibility and stability, and is a supplement for quality inspection of the conventional corn steep liquor.

Description

technical field [0001] The invention belongs to the field of analysis of industrial fermentation raw materials, and relates to a method for finding quality control indexes of corn steep liquor as raw material for fermentation. Background technique [0002] Corn steep liquor is a by-product obtained when steeping corn during the production of cornstarch. It contains amino acids, organic acids, soluble sugars, vitamins and metal salts and other nutrients. It is an important raw material in the production of antibiotics and other culture media, and provides a natural source of organic nitrogen for microbial growth. Because of its low price and rich nutrition, it has been widely used in industrial microbial fermentation. [0003] The existing technical quality testing methods for corn steep liquor are regulated by each manufacturer, and there is no national uniform standard. The current quality inspection stipulates that the appearance is thick and opaque; the color is brown t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/72
Inventor 元英进高赟
Owner TIANJIN UNIV
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