Tumor cell microsatellite instable state complex amplification system and detection kit

Abstract

The invention relates to a tumor cell microsatellite instable state complex amplification system and a detection kit and belongs to the field of biotechnology. In the invention, 8 quasi-monomorphic nucleotide repeated loci with high sensitivity and specificity (including NR-21, NR-22, NR-24, NR-27, BAT-25, BAT-26, MONO-27 and CAT-25) are selected to detect microsatellite instability (MSI). Meanwhile, an amelo (AMEL) is added and a 9-loci multiplex amplification system is constructed, so the result is more accurate. The addition of the gender determining locus can prevent an experiment operator from mixing up samples and delivering wrong results to a certain extent. In the invention, the operation is simple, and large-scale promotion is convenient. With the success of the development of the kit, the method can be used for batch production; the use conditions of the method can be modularized; automatic equipment is applied in the whole process, so the whole process is independent of theoperational experiment of people; and the total detection time is about 6 hours.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a multiple amplification system and a detection kit for disease diagnosis, in particular to a multiple amplification system and a detection kit for tumor cell microsatellite instability, which can quickly predict the occurrence of tumors. Background technique [0002] Colorectal cancer (CRC) is a common malignant tumor that occurs in the colon or rectum. In the past 20 years, the incidence of colorectal cancer (mainly colon cancer) in most countries in the world has been on the rise. The rising trend of the incidence of colorectal cancer in China is also very obvious. The age of onset in China is mostly 40-60 years old, and the peak is around 50 years old, but colorectal cancer patients under 30 years old are not uncommon. An important feature of this disease in China is that the median age of onset of colorectal cancer is about ten years earlier in China than in Europe and the United...

AI Technical Summary

Problems solved by technology

[0016] Finding quasi-monomorphic sites with high sensitivity and specificity for detecting MSI is a necessary work to establish an MSI detection system, and has become a hot spot in the field of MSI detection in recent years, but this work is not easy. So far Only one product from Promega can detect 5 at a time, and it is imperative to develop an MSI detection system with more sites and better sensitivity

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